ProSci

AMPK2 Antibody

Product Code:
 
PSI-62-890
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:1000 dilution + 293 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis of AMPK2 Antibody in Hela cell line lysates (35ug/lane)
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Western Blot at 1:1000 dilution + 293 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of AMPK2 Antibody in Hela cell line lysates (35ug/lane)

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PSI-62-890-400ul400ul£626.00
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Further Information

Additional Names:
5'-AMP-activated protein kinase catalytic subunit alpha-2, AMPK subunit alpha-2, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, PRKAA2, AMPK, AMPK2
Application Note:
For WB starting dilution is: 1:1000
Background:
AMPK2 is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
Background References:
  • Wyatt,C.N., J. Biol. Chem. 282 (11), 8092-8098 (2007)
  • Cheung,S.T., Neoplasia(9), 696-701 (2006)
  • Lee-Young,R.S., Am. J. Physiol. Endocrinol. Metab. 291 (3), E566-E573 (2006)
  • Gregory,S.G., Nature 441 (7091), 315-321 (2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Pig, Rat
Immunogen:
This AMPK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-294 amino acids from the Central region of human AMPK2.
NCBI Gene ID #:
5563
NCBI Official Name:
5'-AMP-activated protein kinase catalytic subunit alpha-2
NCBI Official Symbol:
PRKAA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
62 kDa
Protein Accession #:
P54646
Protein GI Number:
20178276
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Obesity,Neuroscience,Signal Transduction
Swissprot #:
P54646
User NOte:
Optimal dilutions for each application to be determined by the researcher.