ProSci

MAP4K3 Antibody

Product Code:
 
PSI-64-153
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of MAP4K3 Antibody in HepG2 cell line lysates (35ug/lane)
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MAP4K3 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of MAP4K3 Antibody in HepG2 cell line lysates (35ug/lane)
MAP4K3 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-64-153-400ul400ul£626.00
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Further Information

Additional Names:
Mitogen-activated protein kinase kinase kinase kinase 3, Germinal center kinase-related protein kinase, GLK, MAPK/ERK kinase kinase kinase 3, MEK kinase kinase 3, MEKKK 3, MAP4K3, RAB8IPL1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
MAP4K3 encodes a member of the Ste20 family of serine/threonine protein kinases. The protein belongs to the subfamily that consists of members, such as germinal center kinase (GCK), that are characterized by an N-terminal catalytic domain and C-terminal regulatory domain. The kinase activity of the encoded protein can be stimulated by UV radiation and tumor necrosis factor-alpha. The protein specifically activates the c-Jun N-terminal kinase (JNK) signaling pathway. Evidence suggests that it functions upstream of mitogen-activated protein kinase kinase kinase 1 (MEKK1). This gene previously was referred to as RAB8-interacting protein-like 1 (RAB8IPL1), but it has been renamed mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3).
Background References:
  • Yan, L., et al. Mol. Cell 37(5):633-642(2010)
  • Ichikawa, S., et al. J. Bone Miner. Res. (2010) In press :
  • Lam, D., et al. Proc. Natl. Acad. Sci. U.S.A. 106(29):11978-11983(2009)
  • Wu, C., et al. Proteomics 7(11):1775-1785(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MAP4K3 antibody is generated from rabbits immunized with a recombinant protein of human MAP4K3.
NCBI Gene ID #:
8491
NCBI Official Name:
Mitogen-activated protein kinase kinase kinase kinase 3
NCBI Official Symbol:
MAP4K3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
101 kDa
Protein Accession #:
Q8IVH8
Protein GI Number:
29427817
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
Q8IVH8
User NOte:
Optimal dilutions for each application to be determined by the researcher.