ProSci

UBE2D4 Antibody

Product Code:
 
PSI-60-294
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of lysate from Jurkat cell line, using UBE2D4 Antibody at 1:1000 at each lane.
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Western blot analysis of lysate from Jurkat cell line, using UBE2D4 Antibody at 1:1000 at each lane.

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PSI-60-294-400ul400ul£626.00
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Further Information

Additional Names:
Ubiquitin-conjugating enzyme E2 D4, HBUCE1, Ubiquitin carrier protein D4, Ubiquitin-protein ligase D4, UBE2D4, UBCH5D
Application Note:
For WB starting dilution is: 1:1000
Background:
UBE2D4 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination.
Background References:
  • Markson, G., et al. Genome Res. 19(10):1905-1911(2009)
  • van Wijk, S.J., et al. Mol. Syst. Biol. 5, 295 (2009) :
  • Lamesch, P., et al. Genomics 89(3):307-315(2007)
  • Colland, F., et al. Genome Res. 14(7):1324-1332(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus
Immunogen:
This UBE2D4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the C-terminal region of human UBE2D4.
NCBI Gene ID #:
51619
NCBI Official Name:
Ubiquitin-conjugating enzyme E2 D4
NCBI Official Symbol:
UBE2D4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
17 kDa
Protein Accession #:
Q9Y2X8
Protein GI Number:
74753478
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q9Y2X8
User NOte:
Optimal dilutions for each application to be determined by the researcher.