ProSci

USP21 Antibody

Product Code:
 
PSI-61-112
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 2
Western Blot at 1:1000 dilution + human placenta lysate Lysates/proteins at 20 ug per lane.
2 / 2
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
❮❯

Western Blot at 1:1000 dilution + human placenta lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

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PSI-61-112-400ul400ul£626.00
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Further Information

Additional Names:
Ubiquitin carboxyl-terminal hydrolase 21, Deubiquitinating enzyme 21, Ubiquitin thioesterase 21, Ubiquitin-specific-processing protease 21, USP21, USP23
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
USP21 is a ubiquitin-specific protease, an enzyme that removes ubiquitin from ubiquitinated proteins. The encoded protein belongs to the C19 peptidase family, also known as family 2 of ubiquitin carboxyl-terminal hydrolases. This protein has been reported to be capable of removing NEDD8 from NEDD8 conjugates.
Background References:
  • Puente, X.S., et al., Nat. Rev. Genet. 4(7):544-558 (2003).
  • Gong, L., et al., J. Biol. Chem. 275(19):14212-14216 (2000).
  • Hillier, L.D., et al., Genome Res. 6(9):807-828 (1996).
  • Smith, T.S., et al., Biochim. Biophys. Acta 1490 (1-2), 184-188 (2000).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This USP21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 406-436 amino acids from the C-terminal region of human USP21.
NCBI Gene ID #:
27005
NCBI Official Name:
Ubiquitin carboxyl-terminal hydrolase 21
NCBI Official Symbol:
USP21
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
63 kDa
Protein Accession #:
Q9UK80
Protein GI Number:
10720334
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle
Swissprot #:
Q9UK80
User NOte:
Optimal dilutions for each application to be determined by the researcher.