ProSci

BLNK Antibody

Product Code:
 
PSI-62-281
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of BLNK Antibody in NCI-H460 cell line lysates (35ug/lane)
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BLNK Antibody IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of BLNK Antibody in NCI-H460 cell line lysates (35ug/lane)
BLNK Antibody IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-62-281-400ul400ul£626.00
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Further Information

Additional Names:
B-cell linker protein, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, Cytoplasmic adapter protein, Src homology 2 domain-containing leukocyte protein of 65 kDa, SLP-65, BLNK, BASH, SLP65
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
BLNK is a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in its gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia.
Background References:
  • Imamura,Y., J. Biol. Chem. 284 (15), 9804-9813 (2009)
  • Kamino,H., Cancer Sci. 99 (12), 2444-2454 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This BLNK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-178 amino acids from the Central region of human BLNK.
NCBI Gene ID #:
29760
NCBI Official Name:
B-cell linker protein
NCBI Official Symbol:
BLNK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50 kDa
Protein Accession #:
Q8WV28
Protein GI Number:
82592659
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology,Signal Transduction
Swissprot #:
Q8WV28
User NOte:
Optimal dilutions for each application to be determined by the researcher.