ProSci

CA14 Antibody

Product Code:
 
PSI-62-807
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of CA14 antibody in A375 cell line lysates (35ug/lane)
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Flow cytometric analysis of A375 cells using CA14 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of CA14 antibody in A375 cell line lysates (35ug/lane)
Flow cytometric analysis of A375 cells using CA14 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-62-807-400ul400ul£626.00
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Further Information

Additional Names:
Carbonic anhydrase 14, Carbonate dehydratase XIV, Carbonic anhydrase XIV, CA-XIV, CA14
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
CA14 belongs a large family of zinc metalloenzymes that catalyze the versible hydration of carbon dioxide. The protein is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII; however, they have different patterns of tissue-specific expression and thus may play different physiologic roles.
Background References:
  • Parkkila,S. Natl. Acad. Sci. U.S.A. 98 (4), 1918-1923 (2001)
  • Fujikawa-Adachi,K., Nishimori,I. Genomics 61 (1), 74-81 (1999)
  • Tarun,A.S., Bryant,B. Chem. Senses 28 (7), 621-629 (2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CA14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-80 amino acids from the N-terminal region of human CA14.
NCBI Gene ID #:
23632
NCBI Official Name:
Carbonic anhydrase 14
NCBI Official Symbol:
CA14
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
38 kDa
Protein Accession #:
Q9ULX7
Protein GI Number:
8928036
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Neuroscience
Swissprot #:
Q9ULX7
User NOte:
Optimal dilutions for each application to be determined by the researcher.