ProSci

CDH9 Antibody

Product Code:
 
PSI-57-657
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of CDH9 using rabbit polyclonal CDH9 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH9 gene (Lane 2).
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Flow cytometric analysis of MCF-7 cells using CDH9 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of CDH9 using rabbit polyclonal CDH9 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH9 gene (Lane 2).
Flow cytometric analysis of MCF-7 cells using CDH9 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-57-657-400ul400ul£626.00
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Further Information

Additional Names:
Cadherin-9, CDH9
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
CDH9 is a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins.
Background References:
  • Shimoyama,Y., Biochem. J. 349 (PT 1), 159-167 (2000)
  • Nollet,F., J. Mol. Biol. 299 (3), 551-572 (2000)
  • Suzuki,S., Cell Regul.(4), 261-270 (1991)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CDH9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 701-729 amino acids from the C-terminal region of human CDH9.
NCBI Gene ID #:
1007
NCBI Official Name:
Cadherin-9
NCBI Official Symbol:
CDH9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
89 kDa
Protein Accession #:
Q9ULB4
Protein GI Number:
119370307
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
Q9ULB4
User NOte:
Optimal dilutions for each application to be determined by the researcher.