MEKK4 Antibody

Product Code:

PSI-57-989

Product Group:

Primary Antibodies

Supplier:

ProSci

Host Type:

Rabbit

Antibody Isotype:

Rabbit Ig

Antibody Clonality:

Polyclonal

Regulatory Status:

RUO

Target Species:

Human

Applications:

Immunohistochemistry- Paraffin Embedded (IHC-P)
Western Blot (WB)

Storage:

Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

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Code	Size	Price

PSI-57-989-400ul

400ul

£626.00

Quantity:

Prices exclude any Taxes / VAT

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Further Information

Additional Names:

Mitogen-activated protein kinase kinase kinase 4, MAP three kinase 1, MAPK/ERK kinase kinase 4, MEK kinase 4, MEKK 4, MAP3K4, KIAA0213, MAPKKK4, MEKK4, MTK1

Application Note:

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

Background:

The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Two alternatively spliced transcripts encoding distinct isoforms have been described.

Background References:

Grucza, R.A., et al. Addict Biol 15(3):346-357(2010) Whitmarsh, A.J., et al. Oncogene 26(22):3172-3184(2007) Aissouni, Y., et al. Biochem. Biophys. Res. Commun. 338(2):808-814(2005) Abell, A.N., et al. J. Biol. Chem. 280(43):35793-35796(2005) Derbyshire, Z.E., et al. Mol. Cell. Biochem. 271 (1-2), 77-90 (2005) :

Buffer:

Supplied in PBS with 0.09% (W/V) sodium azide.

Concentration:

batch dependent

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Homology:

Predicted species reactivity based on immunogen sequence: Mouse

Immunogen:

This MEKK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1067-1097 amino acids from the Central region of human MEKK4.

NCBI Gene ID #:

4216

NCBI Official Name:

Mitogen-activated protein kinase kinase kinase 4

NCBI Official Symbol:

MAP3K4

NCBI Organism:

Homo sapiens

Physical State:

Liquid

PREDICTED MOLECULAR WEIGHT:

182 kDa

Protein Accession #:

Q9Y6R4

Protein GI Number:

296434576

Purification:

This antibody is purified through a protein A column, followed by peptide affinity purification.

Research Area:

Neuroscience,Signal Transduction

Swissprot #:

Q9Y6R4

User NOte:

Optimal dilutions for each application to be determined by the researcher.

MEKK4 Antibody

Further Information

Documents