ProSci

FFAR3 Antibody

Product Code:
 
PSI-61-840
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of FFAR3 Antibody in Jurkat cell line lysates (35ug/lane)
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Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of FFAR3 Antibody in Jurkat cell line lysates (35ug/lane)
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-61-840-400ul400ul£626.00
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Further Information

Additional Names:
Free fatty acid receptor 3, G-protein coupled receptor 41, FFAR3, GPR41
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
FFAR3 is receptor for short chain fatty acids. The activity of this receptor is coupled to the formation of inositol 1,4,5-trisphosphate, intracellular Ca2+ mobilization, the activation of ERK 1/2 and inhibition of intracellular cAMP accumulation.
Background References:
  • Liaw, C.W., et al. DNA Cell Biol. 28(11):555-560(2009)
  • Tazoe, H., et al. Biomed. Res. 30(3):149-156(2009)
  • Stoddart, L.A., et al. J. Biol. Chem. 283(47):32913-32924(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This FFAR3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293-322 amino acids from the C-terminal region of human FFAR3.
NCBI Gene ID #:
2865
NCBI Official Name:
Free fatty acid receptor 3
NCBI Official Symbol:
FFAR3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
39 kDa
Protein Accession #:
O14843
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Growth Factor
Swissprot #:
O14843
User NOte:
Optimal dilutions for each application to be determined by the researcher.