ProSci

PYCR1 Antibody

Product Code:
 
PSI-64-173
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western blot analysis in K562,WiDr,HepG2 cell line lysates (35ug/lane).
2 / 3
PYCR1 Antibody IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3
❮❯

Western blot analysis in K562,WiDr,HepG2 cell line lysates (35ug/lane).
PYCR1 Antibody IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-64-173-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Additional Names:
Pyrroline-5-carboxylate reductase 1, mitochondrial, P5C reductase 1, P5CR 1, PYCR1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion.
Background References:
  • Reversade, B., et al. Nat. Genet. 41(9):1016-1021(2009)
  • Guernsey, D.L., et al. Am. J. Hum. Genet. 85(1):120-129(2009)
  • Meng, Z., et al. J. Mol. Biol. 359(5):1364-1377(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PYCR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291-319 amino acids from the C-terminal region of human PYCR1.
NCBI Gene ID #:
5831
NCBI Official Name:
Pyrroline-5-carboxylate reductase 1, mitochondrial
NCBI Official Symbol:
PYCR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
33 kDa
Protein Accession #:
P32322
Protein GI Number:
60416434
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
P32322
User NOte:
Optimal dilutions for each application to be determined by the researcher.