ProSci

ERK3 Antibody

Product Code:
 
PSI-62-953
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:500 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:500 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane.

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PSI-62-953-400ul400ul£626.00
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Further Information

Additional Names:
Mitogen-activated protein kinase 6, MAP kinase 6, MAPK 6, Extracellular signal-regulated kinase 3, ERK-3, MAP kinase isoform p97, p97-MAPK, MAPK6, ERK3, PRKM6
Application Note:
For WB starting dilution is: 1:500
Background:
ERK3 is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.
Background References:
  • Coulombe, P., et al., Mol. Cell. Biol. 23(13):4542-4558 (2003).
  • Meloche, S., et al., Oncogene 13(7):1575-1579 (1996).
  • Cheng, M., et al., J. Biol. Chem. 271(15):8951-8958 (1996).
  • Zhu, A.X., et al., Mol. Cell. Biol. 14(12):8202-8211 (1994).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ERK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 692~721 amino acids from the C-terminal region of human ERK3.
NCBI Gene ID #:
5597
NCBI Official Name:
Mitogen-activated protein kinase 6
NCBI Official Symbol:
MAPK6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
83 kDa
Protein Accession #:
Q16659
Protein GI Number:
2499596
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q16659
User NOte:
Optimal dilutions for each application to be determined by the researcher.