ProSci

ILK Antibody

Product Code:
 
PSI-63-101
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ILK Antibody (S246) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of anti-ILK Antibody (S246) in Hela cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ILK Antibody (S246) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-ILK Antibody (S246) in Hela cell line lysates (35ug/lane)

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PSI-63-101-400ul400ul£626.00
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Further Information

Additional Names:
Integrin-linked protein kinase, 59 kDa serine/threonine-protein kinase, ILK-1, ILK-2, p59ILK, ILK, ILK1, ILK2
Application Note:
For IHC-P starting dilution is: 1:50~100

For WB starting dilution is: 1:1000
Background:
Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. ILK is a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction.
Background References:
  • Li, Y., et al., J. Clin. Invest. 112(4):503-516 (2003).
  • Troussard, A.A., et al., J. Biol. Chem. 278(25):22374-22378 (2003).
  • Marotta, A., et al., Br. J. Cancer 88(11):1755-1762 (2003).
  • Cordes, N., et al., Br. J. Cancer 88(9):1470-1479 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This ILK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-253 amino acids from human ILK.
NCBI Gene ID #:
3611
NCBI Official Name:
Integrin-linked protein kinase
NCBI Official Symbol:
ILK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
51 kDa
Protein Accession #:
Q13418
Protein GI Number:
9973397
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q13418
User NOte:
Optimal dilutions for each application to be determined by the researcher.