ProSci

DAAM1 Antibody

Product Code:
 
PSI-61-450
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of DAAM1 using rabbit polyclonal DAAM1 Antibody(Human C-term) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DAAM1 gene (Lane 2).
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Formalin-fixed and paraffin-embedded human brain tissue reacted with DAAM1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of DAAM1 using rabbit polyclonal DAAM1 Antibody(Human C-term) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DAAM1 gene (Lane 2).
Formalin-fixed and paraffin-embedded human brain tissue reacted with DAAM1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-61-450-400ul400ul£626.00
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Further Information

Additional Names:
Disheveled-associated activator of morphogenesis 1, DAAM1, KIAA0666
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. DAAM1 contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by DAAM1, which is thought to function as a scaffolding protein.
Background References:
  • Liu,W., Proc. Natl. Acad. Sci. U.S.A. 105 (1), 210-215 (2008)
  • Yamashita,M.,Genes Cells 12 (11), 1255-1265 (2007)
  • Lu,J., J. Mol. Biol. 369 (5), 1258-1269 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This DAAM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1034-1063 amino acids from the C-terminal region of human DAAM1.
NCBI Gene ID #:
23002
NCBI Official Name:
Disheveled-associated activator of morphogenesis 1
NCBI Official Symbol:
DAAM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
123 kDa
Protein Accession #:
Q9Y4D1
Protein GI Number:
34098767
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9Y4D1
User NOte:
Optimal dilutions for each application to be determined by the researcher.