ProSci

TCEA3 Antibody

Product Code:
 
PSI-58-504
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in HepG2 cell line lysates (35ug/lane).
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Western blot analysis in HepG2 cell line lysates (35ug/lane).

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PSI-58-504-400ul400ul£626.00
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Further Information

Additional Names:
Transcription elongation factor A protein 3, Transcription elongation factor S-II protein 3, Transcription elongation factor TFIISh, TCEA3, TFIISH
Application Note:
For WB starting dilution is: 1:1000
Background:
TCEA3 is necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
Background References:
  • Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
  • Kim, J., et al. Cell 140(4):491-503(2010)
  • Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
  • Labhart, P., et al. Genomics 52(3):278-288(1998)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This TCEA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-130 amino acids from the Central region of human TCEA3.
NCBI Gene ID #:
6920
NCBI Official Name:
Transcription elongation factor A protein 3
NCBI Official Symbol:
TCEA3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
39 kDa
Protein Accession #:
O75764
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
O75764
User NOte:
Optimal dilutions for each application to be determined by the researcher.