ProSci

DDX6 Antibody

Product Code:
 
PSI-64-175
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in Hela,MCF-7 cell line lysates (35ug/lane).
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DDX6 Antibody IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in Hela,MCF-7 cell line lysates (35ug/lane).
DDX6 Antibody IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-64-175-400ul400ul£626.00
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This product comes from: United States.
Typical lead time: 14-21 working days.
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Further Information

Additional Names:
Probable ATP-dependent RNA helicase DDX6, ATP-dependent RNA helicase p54, DEAD box protein 6, Oncogene RCK, DDX6, HLR2, RCK
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing.
Background References:
  • Broytman, O., et al. Neurobiol. Aging 30(12):1962-1974(2009)
  • Han, J.W., et al. Nat. Genet. 41(11):1234-1237(2009)
  • Minshall, N., et al. Mol. Biol. Cell 20(9):2464-2472(2009)
  • Tritschler, F., et al. Mol. Cell 33(5):661-668(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Mouse, Xenopus
Immunogen:
This DDX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 348-375 amino acids from the Central region of human DDX6.
NCBI Gene ID #:
1656
NCBI Official Name:
Probable ATP-dependent RNA helicase DDX6
NCBI Official Symbol:
DDX6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
54 kDa
Protein Accession #:
P26196
Protein GI Number:
116241327
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P26196
User NOte:
Optimal dilutions for each application to be determined by the researcher.