ProSci

PBP Antibody

Product Code:
 
PSI-63-392
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of anti-PBP Antibody Antibody in 293 cell line lysates (35ug/lane)
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Western blot analysis of anti-PBP Antibody in mouse NIH-3T3 tissue lysates (35ug/lane)
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Western blot analysis of PBP using rabbit polyclonal PBP Antibody (A152) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PBP gene.
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Western blot analysis of anti-PBP Antibody Antibody in 293 cell line lysates (35ug/lane)
Western blot analysis of anti-PBP Antibody in mouse NIH-3T3 tissue lysates (35ug/lane)
Western blot analysis of PBP using rabbit polyclonal PBP Antibody (A152) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PBP gene.

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PSI-63-392-400ul400ul£626.00
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Further Information

Additional Names:
Phosphatidylethanolamine-binding protein 1, PEBP-1, HCNPpp, Neuropolypeptide h3, Prostatic-binding protein, Raf kinase inhibitor protein, RKIP, Hippocampal cholinergic neurostimulating peptide, HCNP, PEBP1, PBP, PEBP
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
PBP binds ATP, opioids and phosphatidylethanolamine, exhibiting a lower affinity for phosphatidylinositol and phosphatidylcholine. This serine protease inhibitor inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase. PBP contains hippocampal cholinergic neurostimulating peptide (HCNP), which may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase.
Background References:
  • Tohdoh, N., et al., Brain Res. Mol. Brain Res. 30(2):381-384 (1995). Hori, N., et al., Gene 140(2):293-294 (1994). Seddiqi, N., et al., J. Mol. Evol. 39(6):655-660 (1994). Moore, C., et al., Brain Res. Mol. Brain Res. 37 (1-2), 74-78 (1996).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PBP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137-167 amino acids from the Central region of human PBP.
NCBI Gene ID #:
5037
NCBI Official Name:
Phosphatidylethanolamine-binding protein 1
NCBI Official Symbol:
PEBP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
21 kDa
Protein Accession #:
P30086
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Neuroscience
Swissprot #:
P30086
User NOte:
Optimal dilutions for each application to be determined by the researcher.