ProSci

Mouse Raf1 Antibody

Product Code:
 
PSI-57-614
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in Jurkat cell line lysates (35ug/lane).
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Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
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Mouse Raf1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in Jurkat cell line lysates (35ug/lane).
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
Mouse Raf1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-57-614-400ul400ul£626.00
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Further Information

Additional Names:
RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, cRaf, Raf-1, Raf1, Craf
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:10~50
Background:
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3 (By similarity).
Background References:
  • Niault, T.S., et al. Carcinogenesis 31(7):1165-1174(2010)
  • Batarseh, A., et al. Biochemistry 49(23):4766-4778(2010)
  • Tarutani, M., et al. J. Dermatol. Sci. 58(1):28-35(2010)
  • Heidorn, S.J., et al. Cell 140(2):209-221(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This Mouse Raf1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-113 amino acids from the N-terminal region of mouse Raf1.
NCBI Gene ID #:
110157
NCBI Official Name:
RAF proto-oncogene serine/threonine-protein kinase
NCBI Official Symbol:
Raf1
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
73 kDa
Protein Accession #:
Q99N57
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Signal Transduction
Swissprot #:
Q99N57
User NOte:
Optimal dilutions for each application to be determined by the researcher.