ProSci

Mouse Nlk Antibody

Product Code:
 
PSI-57-359
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstratedetected the Nlk protein (arrow).
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Western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstratedetected the Nlk protein (arrow).
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Western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstratedetected the Nlk protein (arrow).
Western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstratedetected the Nlk protein (arrow).

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PSI-57-359-400ul400ul£626.00
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Further Information

Additional Names:
Serine/threonine-protein kinase NLK, Nemo-like kinase, Nlk {ECO:0000312|EMBL:AAC244991}
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP. Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3.
Background References:
  • Li, M., et al. J. Biol. Chem. 285(18):13397-13404(2010)
  • Nifuji, A., et al. Exp. Cell Res. 316(7):1127-1136(2010)
  • Ishitani, T., et al. Nat. Cell Biol. 12(3):278-285(2010)
  • Martinez, G., et al. Invest. Ophthalmol. Vis. Sci. 50(10):4794-4806(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This Mouse Nlk antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 408-436 amino acids from the C-terminal region of mouse Nlk.
NCBI Gene ID #:
18099
NCBI Official Name:
Serine/threonine-protein kinase NLK
NCBI Official Symbol:
Nlk
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
58 kDa
Protein Accession #:
O54949
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
O54949
User NOte:
Optimal dilutions for each application to be determined by the researcher.