ProSci

SPCS3 Antibody

Product Code:
 
PSI-62-393
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of SPCS3 antibody in mouse liver tissue lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SPCS3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of SPCS3 antibody in mouse liver tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SPCS3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-62-393-400ul400ul£626.00
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Further Information

Additional Names:
Signal peptidase complex subunit 3, 34--, Microsomal signal peptidase 22/23 kDa subunit, SPC22/23, SPase 22/23 kDa subunit, SPCS3, SPC22
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
SPCS3 is a component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum.
Background References:
  • Clark,H.F., Genome Res. 13 (10), 2265-2270 (2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This SPCS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-180 amino acids from the C-terminal region of human SPCS3.
NCBI Gene ID #:
60559
NCBI Official Name:
Signal peptidase complex subunit 3
NCBI Official Symbol:
SPCS3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
20 kDa
Protein Accession #:
P61009
Protein GI Number:
46577648
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
P61009
User NOte:
Optimal dilutions for each application to be determined by the researcher.