ProSci

FMOD Antibody

Product Code:
 
PSI-63-852
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of FMOD Antibody in mouse cerebellum tissue lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human brain tissue reacted with FMOD Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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❮❯

Western blot analysis of FMOD Antibody in mouse cerebellum tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with FMOD Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-63-852-400ul400ul£626.00
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Further Information

Additional Names:
Fibromodulin, FM, Collagen-binding 59 kDa protein, Keratan sulfate proteoglycan fibromodulin, KSPG fibromodulin, FMOD, FM, SLRR2E
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Fibromodulin is a member of a family of small interstitial proteoglycans, containing a central region composed of leucine-rich repeats with 4 keratan sulfate chains flanked by disulfide-bonded terminal domains. It may participate in the assembly of the extracellular matrix as it interacts with type I and type II collagen fibrils and inhibits fibrillogenesis in vitro. It may also regulate TGF-beta activities by sequestering TGF-beta into the extracellular matrix.
Background References:
  • Ehret,G.B., et.al, Eur. J. Hum. Genet. 17 (12), 1650-1657 (2009)
  • Tillgren,V., et.al, J. Biol. Chem. 284 (42), 28543-28553 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This FMOD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 337-364 amino acids from the C-terminal region of human FMOD.
NCBI Gene ID #:
2331
NCBI Official Name:
Fibromodulin
NCBI Official Symbol:
FMOD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
43 kDa
Protein Accession #:
Q06828
Protein GI Number:
223590208
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q06828
User NOte:
Optimal dilutions for each application to be determined by the researcher.