ProSci

Glycoprotein / GP (virus) Recombinant Protein

Product Code:
 
PSI-97-050
Product Group:
 
Recombinant Proteins
Supplier:
 
ProSci
Regulatory Status:
 
RUO
Target Species:
 
Ebola Virus (EBOV)
Application:
 
Western Blot (WB)
Storage:
 
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
 

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PSI-97-050-0.05mg0.05mg£657.00
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Further Information

Additional Names:
Ebolavirus Glycoprotein
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160-kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.
Background References:
  • Saeed M.F., et al., 2010, PLoS Pathog. 6:0-0.
  • Marzi A., et al., 2006, J. Virol. 80:6305-6317.
  • Chandran K., et al., 2005, Science 308:1643-1645.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
9487265
NCBI Official Name:
spike glycoprotein
NCBI Official Symbol:
Glycoprotein / GP (virus)
NCBI Organism:
Bundibugyo ebolavirus
Physical State:
Lyophilized
Predicted Molecular Weight:
34.3 kDa
Protein Accession #:
B8XCN1
Purity:
>95% as determined by SDS-PAGE.

Endotoxin level is less than 1.0 EU per ug by the LAL method.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
B8XCN1

Documents