ProSci

CTXB Recombinant Protein

Product Code:
 
PSI-97-073
Product Group:
 
Recombinant Proteins
Supplier:
 
ProSci
Regulatory Status:
 
RUO
Target Species:
 
Vibrio species (bacteria)
Application:
 
Western Blot (WB)
Storage:
 
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
 

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PSI-97-073-0.05mg0.05mg£686.00
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Further Information

Additional Names:
CTXB,TOXB
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Cholera enterotoxin subunit B (CTXB) is also known as cholera enterotoxin B chain, cholera enterotoxin gamma chain and choleragenoid. Vibrio cholerae O1 exists as two major serotypes, Inaba and Ogawa, which are associated with the O antigen of the lipopolysaccharide and are capable of unequal reciprocal interconversion. The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. CTXB can bind five GM1 gangliosides. Also, it has no toxic activity by itself.
Background References:
  • Lockman H., et al., 1983, J. Biol. Chem. 258:13722-13726.
  • Fujinaga Y., et al., 2003, Mol. Biol. Cell 14:4783-4793.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
2613962
NCBI Official Name:
Cholera enterotoxin subunit B
NCBI Official Symbol:
CTXB
NCBI Organism:
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Physical State:
Lyophilized
Predicted Molecular Weight:
13.5 kDa
Protein Accession #:
P01556
Purity:
>92% as determined by SDS-PAGE.

Endotoxin level is less than 1.0 EU per ug by the LAL method.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P01556

Documents