ProSci

UCH-L3 Recombinant Protein

Product Code:
 
PSI-96-960
Product Group:
 
Recombinant Proteins
Supplier:
 
ProSci
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
 

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PSI-96-960-0.1mg0.1mg£626.00
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Further Information

Additional Names:
UCHL3,Ubiquitin thioesterase L3
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Ubiquitin carboxyl-terminal hydrolase isozyme L3 (UCH-L3), a member of peptidase C12 family, is also known as ubiquitin thioesterase L3. Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. UCH-L3 is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCH-L3 indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is also required for stress-response retinal, skeletal muscle and germ cell maintenance. Furthermore, UCH-L3 may be involved in working memory and can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders.
Background References:
  • Nam M.J., et al., 2003, Proteomics 3:2108-2115.
  • Setsuie R., et al., 2009, Neurochem. Int. 54:314-321.
  • Iphofer A., et al., 2012, ChemBioChem 13:1416-1420.
Buffer:
50 mM Tris, 150 mM NaCl, pH7.5
Fusion Tag:
His Tag
NCBI Gene ID #:
7347
NCBI Official Name:
Ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
NCBI Official Symbol:
UCH-L3
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
27 kDa
Protein Accession #:
AAH18125
Purity:
>95% as determined by SDS-PAGE.

Endotoxin level is less than 1.0 EU per ug by the LAL method.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
NP_005993.1

Documents