Nampt (mouse) (rec.) (enzymatically active)

Visfatin; PBEF; Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase; NAmPRTase

1µg to 5µg of Nampt (mouse) (rec.) (enzymatically active) show activity in a colorimetric Nampt assay.

Lot dependent.

32160000

<0.01EU/µg purified protein (LAL test).

liquid

Liquid. Contains PBS.

After opening, prepare aliquots and store at -80°C.Avoid freeze/thaw cycles.

Protein. Mouse Nampt (visfatin/PBEF) (aa 1-491) is fused at the N-terminus to a FLAG®-tag. Source: HEK 293 cells. Endotoxin content: <0.01EU/µg purified protein (LAL test; Lonza). Liquid. Contains PBS. Purity: >90% (SDS-PAGE). Nicotinamide phosphoribosyltransferase (NAmPRTase or Nampt) also known as pre-B cell colony-enhancing factor 1 (PBEF1) or visfatin is the rate-limiting enzyme in the nicotinamide adenine dinucleotide (NAD+) salvage pathway that converts nicotinamide to nicotinamide mononucleotide in mammals to enable NAD+ biosynthesis. In addition it has been shown to be an adipokine that is localized in the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. The recombinant Nampt (mouse) (rec.) (enzymatically active) (Prod. No. AG-40B-0179) shows activity to convert nicotinamide + phosphoribosyltransferase (PRPP) to nicotinamide mononucleotide (NMN). It migrates as a homodimer on a size exclusion chromatography (SEC) column.

~55kDa (SDS-PAGE); ~100-110 kDa (Dimer) (SEC)

Q99KQ4

Plastic Vial

Nicotinamide phosphoribosyltransferase (NAmPRTase or Nampt) also known as pre-B cell colony-enhancing factor 1 (PBEF1) or visfatin is the rate-limiting enzyme in the nicotinamide adenine dinucleotide (NAD+) salvage pathway that converts nicotinamide to nicotinamide mononucleotide in mammals to enable NAD+ biosynthesis. In addition it has been shown to be an adipokine that is localized in the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. The recombinant Nampt (mouse) (rec.) (enzymatically active) (Prod. No. AG-40B-0179) shows activity to convert nicotinamide + phosphoribosyltransferase (PRPP) to nicotinamide mononucleotide (NMN). It migrates as a homodimer on a size exclusion chromatography (SEC) column.

>90% (SDS-PAGE)

Mouse Nampt (visfatin/PBEF) (aa 1-491) is fused at the N-terminus to a FLAG®-tag.

HEK 293 cells

FLAG

Non-hazardous

Other Proteins

12352202

Stable for at least 6 months after receipt when stored at -80°C. Working aliquots are stable for up to 3 months when stored at -20°C.