NSJ Bioreagents

BRAF Antibody (B Raf)

Product Code:
 
NSJ-R31582
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application:
 
Western Blot (WB)
Storage:
 
The lyophilized BRAF antibody can be stored at 40 to -200. After reconstitution, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 8
IHC staining of FFPE human placental tissue with BRAF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 8
IHC staining of FFPE human adenocarcinoma of the rectum with BRAF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 8
IHC staining of FFPE human lung cancer tissue with BRAF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 8
IHC staining of FFPE human liver cancer tissue with BRAF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 8
IHC staining of FFPE mouse brain tissue with BRAF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 8
IHC staining of FFPE rat brain tissue with BRAF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 8
Western blot testing of 1) human SH-SY5Y, 2) rat brain, 3) rat testis and 4) mouse brain lysate with BRAF antibody. Expected molecular weight: 85-95 kDa.
8 / 8
Flow cytometry testing of human U-2 OS cells with BRAF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BRAF antibody.
❮❯

IHC staining of FFPE human placental tissue with BRAF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human adenocarcinoma of the rectum with BRAF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with BRAF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with BRAF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with BRAF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with BRAF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human SH-SY5Y, 2) rat brain, 3) rat testis and 4) mouse brain lysate with BRAF antibody. Expected molecular weight: 85-95 kDa.
Flow cytometry testing of human U-2 OS cells with BRAF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BRAF antibody.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-R31582-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 2-5ug/ml,Flow cytometry: 1-3ug/million celss
Application Note:
Titration of the BRAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
BRAF (v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a protein called B Raf. It is a member of the Raf kinase family of growth signal transduction protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. It is mapped to 7q34. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. The BRAF protein is also involved in sending signals inside cells, which are involved in directing cell growth.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
673
Immunogen:
Human partial recombinant protein (AA 38-230) was used as the immunogen for this BRAF antibody.
Limitation:
This BRAF antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat