NSJ Bioreagents

Paxillin Antibody

Product Code:
 
NSJ-R30070
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PXC-10, aka PAX-14
Regulatory Status:
 
RUO
Target Species:
  • Chicken (Gallus)
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Paxillin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
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Western blot testing of Paxillin antibody and Lane 1: 293T; 2: HeLa; 3: MCF-7; 4: MM231; 5: Jurkat cell lysate
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Western blot testing of Paxillin antibody and Lane 1: 293T; 2: HeLa; 3: MCF-7; 4: MM231; 5: Jurkat cell lysate

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NSJ-R30070-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 1-2ug/ml,Immunocytochemistry: Suitable
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Paxillin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The paxillin gene can be alternatively spliced to include 1 of 2 alternative exons, generating beta and gamma isoforms. Paxillin is a 68-kDa focal adhesion protein that is phosphorylated on tyrosine residues in fibroblasts in response to transformation by v-src, treatment with platelet-derived growth factor, or cross-linking of integrins. The 68-kD protein(paxillin) is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers in chicken embryo fibroblasts. It is also present in the focal adhesions of Madin-Darby bovine kidney(MDBK) epithelial cells but is absent, like talin, from the cell-cell adherens junctions of these cells.
Format :
Ascites
Formulation :
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Gene ID #:
5829
Immunogen:
C-terminal partial recombinant chicken paxillin (amino acids 305-559) was used as the immunogen for this Paxillin antibody.
Limitation:
This Paxillin antibody is available for research use only.
Purity:
Ascites
Species Reactivity :
Human, mouse, rat, chicken