Chimerigen

TGFbeta1 (mutant) (human):Fc (human) (rec.)

Product Code:
 
CHI-HF-210TGFBM
Product Group:
 
Recombinant Proteins
Supplier:
 
Chimerigen
Host Type:
 
Hamster
Regulatory Status:
 
RUO
Target Species:
 
Human
Shipping:
 
BI
Storage:
 
-20°C
 

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CHI-HF-210TGFBM-C100100 ug£465.00
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This product comes from: Switzerland.
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Further Information

Alternate Names/Synonyms:
Transforming Growth Factor beta-1; TGFB1; TGFB
Biological Activity:
Shows the biological function of the TGFbeta1 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain.
EClass:
32160000
Endotoxin:
<0.06EU/µg protein (LAL test; Lonza).
Form (Short):
solid
Formulation:
Lyophilized from 0.2µm-filtered solution in PBS.
Handling Advice:
Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.
Long Description:
Protein. The extracellular domain of a mutant human TGFbeta1 is fused to the N-terminus of the Fc region of human IgG4. Site-directed mutagenesis was used to change three cysteine codons into a serine codon that are located in the pro region of the TGF-beta precursor at amino acid positions 33, 223, and 225. Source: CHO cells. Endotoxin content: <0.06EU/µg protein (LAL test; Lonza). Lyophilized from 0.2µm-filtered solution in PBS. Binds human TGFbeta. Shown to cross-react with mouse TGFbeta (see lit. reference 1). Purity: >98% (SDS-PAGE). TGF-beta1 (Transforming growth factor beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. In humans, TGF-beta1 is encoded by the TGFB1 gene. TGF-beta1 was first identified in human platelets as a protein with a molecular mass of 25 kaD with a potential role in wound healing. It was later characterized as a large protein precursor (containing 390 aa) that was proteolytically processed to produce a mature peptide of 112 aa. TGF-beta1 plays an important role in controlling the immune system, and shows different activities on different types of cell, or cells at different developmental stages. Most immune cells (or leukocytes) secrete TGF-beta1.
Package Type:
Plastic Vial
Product Description:
TGF-beta1 (Transforming growth factor beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. In humans, TGF-beta1 is encoded by the TGFB1 gene. TGF-beta1 was first identified in human platelets as a protein with a molecular mass of 25 kaD with a potential role in wound healing. It was later characterized as a large protein precursor (containing 390 aa) that was proteolytically processed to produce a mature peptide of 112 aa. TGF-beta1 plays an important role in controlling the immune system, and shows different activities on different types of cell, or cells at different developmental stages. Most immune cells (or leukocytes) secrete TGF-beta1. Normally, TGF-beta1 is secreted as a complex with Latency-Associated Peptide (LAP) that is inactive. Removal of LAP activates TGF-beta1 that is cleared quickly from the circulation. This specific mutant protein containing the LAP+TGF-beta1 domains including the indicated three mutated cysteines, allows the protein to be active and to have long lasting biological activity with increased stability and half-life time.
Purity:
>98% (SDS-PAGE)
Sequence:
The mutant human TGF-beta1 (including aa30-278 (Latency-Associated Peptide, LAP) and aa279-390 (TGF-beta1)) is fused to the N-terminus of the Fc region of human IgG4. Site-directed mutagenesis was used to change three cysteine codons into a serine codon that are located in the pro region of the TGF-beta precursor at amino acid positions 33, 223, and 225.
Source / Host:
CHO cells
Specificity:
Binds human TGFbeta. Shown to cross-react with mouse TGFbeta (see lit. reference 1).
TAGs:
Fc
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

References

Combined administration of a mutant TGF-beta1/Fc and rapamycin promotes induction of regulatory T cells and islet allograft tolerance: W. Zhang, et al.; J. Immunol. 185, 4750 (2010)