Welcome to 3Helix - Distributed in the UK & Europe by Caltag Medsystems

Collagen Hybridising Peptide (CHP) is a synthetic peptide that specifically binds to unfolded collagen molecules by forming the same triple helical structure with the denatured collagen chains. This is similar to a primer binding to a melted DNA strand during PCR, enabling unlimited laboratory applications in pathology, biomechanics, regenerative medicine, and more.

• More informative, reliable and convenient than zymography, DQ collagen, SHG, and TEM
• High affinity and unparalleled specificity to collagen with essentially no nonspecific binding
• Applicable to all types of collagen from all species, relying on collagen’s secondary structure instead of any defined sequence for binding
• Suitable for both frozen and paraffin-embedded sections with no need for antigen retrieval
• A non-antibody approach with no species restrictions against any co-staining antibody
• Small size (2% of IgG by MW) enabling facile tissue penetration and whole specimen staining without sectioning
• Stable in solution under 4 °C, eliminating the need to aliquot for storage

1. Histopathology - CHP marks tissue damage and remodelling under many pathologic and physiologic events through binding to degraded collagen.
2. Collagen Identification - CHP visualises collagen bands of all types in SDSPAGE with high specificity.
3. Mechanical Damage - CHP enables measuring and localising mechanical injury to collagenous tissue at the molecular level in almost all possible tissue types, including bone, cartilage, tendon, ligament, intervertebral discs, heart valves, blood vessels, skin, cornea and more.
4. Tissue Decellularisation - CHP allows direct and quantitative assessment of denatured collagen in the ECM materials decellularized with different detergents and protocols.