Welcome to 3Helix - Distributed in the UK & Europe by Caltag Medsystems

The collagen hybridizing peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains. By sharing the Gly-X-Y repeating sequence of natural collagen, CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure, in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has a negligible affinity for intact collagen molecules due to its lack of binding sites, and it is inert towards non-specific binding because of its neutral and hydrophilic nature.

Their new product line, the sulfo-Cy7.5-CHP conjugates, are the perfect tool for in vivo detection of collagen degradation. This new sequence allows the peptide to be directly injected into animals without having to pre-activate them with a heating step. Therefore, the in vivo CHPs will always be in their active form and will not self-hybridize once injected. The conjugation of the peptide to the sulfonated-cyanine 7.5 dye allows near-infrared fluorescence detection, which offers better tissue penetration while minimizing tissue autofluorescence.

• More informative, reliable and convenient than zymography, DQ collagen, SHG, and TEM
• High affinity and unparalleled specificity to collagen with essentially no nonspecific binding
• Applicable to all types of collagen from all species, relying on collagen’s secondary structure instead of any defined sequence for binding
• Suitable for both frozen and paraffin-embedded sections with no need for antigen retrieval
• A non-antibody approach with no species restrictions against any co-staining antibody
• Small size (2% of IgG by MW) enabling facile tissue penetration and whole specimen staining without sectioning
• Stable in solution under 4 °C, eliminating the need to aliquot for storage
• Now suitable for in vivo studies

1. Histopathology - CHP marks tissue damage and remodelling under many pathologic and physiologic events through binding to degraded collagen.
2. Collagen Identification - CHP visualises collagen bands of all types in SDSPAGE with high specificity.
3. Mechanical Damage - CHP enables measuring and localising mechanical injury to collagenous tissue at the molecular level in almost all possible tissue types, including bone, cartilage, tendon, ligament, intervertebral discs, heart valves, blood vessels, skin, cornea and more.
4. Tissue Decellularisation - CHP allows direct and quantitative assessment of denatured collagen in the ECM materials decellularized with different detergents and protocols.