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3Helix

Welcome to 3Helix - Distributed in the UK & Europe by Caltag Medsystems

3Helix has developed a series of proprietary collagen hybridizing peptides that directly target the damaged collagen molecule. Collagen is the major component of nearly every human tissue, with an essential role in supporting cell growth and tissue formation. Damage to collagen is a strong indication of connective tissue injury, as well as a variety of diseases involving inflammation and abnormal tissue remodelling, such as cancer, myocardial infarction, arthritis, osteoporosis, and fibrosis.

In addition to providing Collagen Hybridising Peptide (CHP) as a research tool for laboratory use in academia and industry, 3Helix is advancing its compounds to enable new pathways to achieving diagnostic and therapeutic objectives.

Products

Collagen Hybridising Peptide (CHP) is a synthetic peptide that specifically binds to unfolded collagen molecules by forming the same triple helical structure with the denatured collagen chains. This is similar to a primer binding to a melted DNA strand during PCR, enabling unlimited laboratory applications in pathology, biomechanics, regenerative medicine, and more.

Name Collagen Hybridising Peptide, 5-FAM Conjugate Collagen Hybridising Peptide, Cy3 Conjugate Collagen Hybridising Peptide, Biotin Conjugate
Product Codes 3H-FLU300 /
3H-FLU60
3H-RED300 /
3H-RED60
3H-BIO300 /
3H-BIO60
Size 300µg / 60µg 300µg / 60µg 300µg / 60µg
Specialty Straightforward fluorescence detection in green Straightforward fluorescence detection in red Flexible avidin / streptavidin mediated detection options based on needs, allowing non-green fluorescence and HRP methods to avoid background and enhance signal
Molecular weight 2952.01 g/mol 3191.44 g/mol 2762.01 g/mol
Purity 95% 90% 95%

Features

  • More informative, reliable and convenient than zymography, DQ collagen, SHG, and TEM
  • High affinity and unparalleled specificity to collagen with essentially no nonspecific binding
  • Applicable to all types of collagen from all species, relying on collagen’s secondary structure instead of any defined sequence for binding
  • Suitable for both frozen and paraffin-embedded sections with no need for antigen retrieval
  • A non-antibody approach with no species restrictions against any co-staining antibody
  • Small size (2% of IgG by MW) enabling facile tissue penetration and whole specimen staining without sectioning
  • Stable in solution under 4 °C, eliminating the need to aliquot for storage
  CHP Collagen antibodies C1, 2C antibody In situ zymography SHG TEM
Detecting denatured collagen  
A direct method        
Applicable to multiple collagen types          
Applicable to mm-sized tissue samples      
No control test needed      
No advanced instrument or specialised skill needed          

Applications

  1. Histopathology - CHP marks tissue damage and remodelling under many pathologic and physiologic events through binding to degraded collagen.
  2. Collagen Identification - CHP visualises collagen bands of all types in SDSPAGE with high specificity.
  3. Mechanical Damage - CHP enables measuring and localising mechanical injury to collagenous tissue at the molecular level in almost all possible tissue types, including bone, cartilage, tendon, ligament, intervertebral discs, heart valves, blood vessels, skin, cornea and more.
  4. Tissue Decellularisation - CHP allows direct and quantitative assessment of denatured collagen in the ECM materials decellularized with different detergents and protocols.

Collagen Mimetic Peptides

Prof. Michael Yu describes examples of the vast potential medical applications of CHP, which was previously termed collagen mimetic peptide.

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