One-component TMB Substrate

Elabscience
Product Code: E-IR-R201
Supplier: Elabscience
CodeSizePrice
E-IR-R201-100mL100mL£119.00
Quantity:
E-IR-R201-500mL500mL£226.00
Quantity:
E-IR-R201-1000mL1000mL£307.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
Ice packs
Storage:
2-8°C/-20°C, 6 months

Documents

Further Information

Usage:
TMB is a popular chromogenic substrate for HRP detection in ELISA and is available in several formats. The soluble One-component TMB Substrate are supplied as Ready-to-Use mono-component liquids that require no preparation before use. The product mainly contains 3,3?,5,5?-tetramethylbenzidine and contains no DMF or DMSO. One-component TMB Substrate yields a blue color (Amax= 370 nm and 652 nm) when reacted with peroxidase in microwell applications. The product changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

References

  • https://www.mdpi.com/article/10.3390/nano12152551
  • https://www.mdpi.com/1012212
  • https://www.mdpi.com/1675836
  • https://portlandpress.com/bioscirep/article-abstract/39/12/BSR20193094/221348
  • https://peerj.com/articles/5056