X-Gal
| Code | Size | Price |
|---|
| AG-CC1-0003-M050 | 50 mg | £32.00 |
Quantity:
| AG-CC1-0003-M250 | 250 mg | £42.00 |
Quantity:
| AG-CC1-0003-G001 | 1 g | £55.00 |
Quantity:
| AG-CC1-0003-G005 | 5 g | £150.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ambient
Storage:
-20°C
Documents
Further Information
Alternate Names/Synonyms:
5-Bromo-4-chloro-3-indolyl beta-D-galactoside
Appearance:
White to off-white powder. Clear, colourless to light yellow in solution.
CAS:
7240-90-6
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Protect from light and moisture.
InChi:
InChI=1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8?,11-,12-,13+,14+/m0/s1
InChiKey:
OPIFSICVWOWJMJ-LMNFNBGQSA-N
Long Description:
Chemical. CAS: 7240-90-6. Formula: C14H15BrClNO6. MW: 408.6. Molecular biology reagent. Chromogenic substrate for beta-galactosidase. Used in gene cloning together with IPTG (Prod. No. AG-CC1-0002) to indicate whether a cell expresses the beta-galactosidase enzyme, which is encoded by the lacZ gene, in a technique called blue/white screening. Distinguishes between recombinant and non-recombinant plasmids carrying the beta-galactosidase gene. Used in two-hybrid analysis. X-Gal is used to determine E. coli and coliform content in drinking water samples.
MDL:
MFCD00005666
Molecular Formula:
C14H15BrClNO6
Molecular Weight:
408.6
Other data:
Optical Rotation [alpha]D20 (c=1,DMF:water, 1:1): -61.5° ? 1.0°Water Content (Karl Fischer): max 1%.
Package Type:
Vial
Product Description:
Molecular biology reagent. Chromogenic substrate for beta-galactosidase. Used in gene cloning together with IPTG (Prod. No. AG-CC1-0002) to indicate whether a cell expresses the beta-galactosidase enzyme, which is encoded by the lacZ gene, in a technique called blue/white screening. Distinguishes between recombinant and non-recombinant plasmids carrying the beta-galactosidase gene. Used in two-hybrid analysis. X-Gal is used to determine E. coli and coliform content in drinking water samples.
Purity:
>99% (HPLC)
SMILES:
OCC1O[C@@H](OC2=CNC3=C2C(Cl)=C(Br)C=C3)C(O)[C@H](O)[C@H]1O
Solubility Chemicals:
Soluble in dimethyl formamide.
Transportation:
Non-hazardous
UNSPSC Category:
Biochemical Reagents
UNSPSC Number:
12352200
Use & Stability:
Stable for at least 3 years after receipt when stored at -20°C.
References
Chemiluminescent assay of various enzymes using indoxyl derivatives as substrate and its applications to enzyme immunoassay and DNA probe assay: H. Arakawa, et al.; Anal. Biochem. 199, 238 (1991) | In situ histochemical detection of beta-galactosidase activity in lung: assessment of X-Gal reagent in distinguishing lacZ gene expression and endogenous beta-galactosidase activity: D.J. Weiss, et al.; Hum. Gene Ther. 8, 1545 (1997) | A novel colorimetric yeast bioassay for detecting trichothecene mycotoxins: K.H. Engler, et al.; J. Microbiol. Methods 35, 207 (1999) | Molecular Cloning: A Laboratory Manual 3rd Edition: J. Sambrook & D. Russell; Cold Spring Harbor Laboratory Press (2001) | Effects of tissue pressure on transgene expression characteristics via renal local administration routes from ureter or renal artery in the rat kidney: N. Oyama, et al.; MDPI Pharmaceutics 12, 114 (2020)
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