AdipoGen Life Sciences

anti-PARP-10 [ARTD10] (human) mAb (5H11)

Product Code:
 
AG-20T-0004
Product Group:
 
Primary Antibodies
Antibody Isotype:
 
Rat IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
5H11
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunocytochemistry (ICC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
Blue Ice
Storage:
 
Short term: +4°C. Long term: -20°C
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Immunofluorescence using anti-PARP-10 [ARTD10] (human), mAb (5H11). Method: T47D mammary carcinoma cells were stained by indirect immunofluorescence and analysed. Prior to fixation and staining, the cells were treated with or without 50nm leptomyci
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Immunofluorescence using anti-PARP-10 [ARTD10] (human), mAb (5H11). Method: T47D mammary carcinoma cells were stained by indirect immunofluorescence and analysed. Prior to fixation and staining, the cells were treated with or without 50nm leptomyci

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AG-20T-0004-L0011 ml£337.00
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Further Information

Alternate Names/Synonyms:
ADP-ribosyltransferase Diphtheria Toxin-like 10; Poly(ADP-ribose) Polymerase-10
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. Cell culture supernatant containing RPMI, 10% FCS and 0.05% sodium azide.
Handling Advice:
After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.
Immunogen:
Recombinant human PARP-10 [ARTD10] (aa 1-907) fused to a GST-tag.
Long Description:
Monoclonal Antibody. Recognizes human PARP-10 [ARTD10] with an epitope between aa 256-407, containing the glycine rich (G-rich) region. Isotype: Rat IgG1. Clone: 5H11. Applications: ICC, IP, WB. Liquid. Cell culture supernatant containing RPMI, 10% FCS and 0.05% sodium azide. PARP-10 is a mono-ADP-ribosyltransferase and was initially identified as an interaction partner of the proto-oncoprotein Myc. PARP-10 possesses ubiquitin-interaction motifs, which are important to inhibit the activation of NF-kappaB and downstream target genes in response to interleukin-1beta and tumor necrosis factor-alpha. PARP-10 mono(ADP-ribosyl)ates (MARylates) NEMO (NF-kappaB essential modulator), which results in reduced NEMO polyubiquitylation and thus decreased NF-kappaB signaling. Overexpression of PARP-10 was shown to lead to apoptosis. PARP-10 knockdown leads to increased cell survival. Additional substrates of PARP-10, such as GSK-3beta were identified and PARP-10-mediated mono(ADP-ribosyl)ation (MARylation) was shown to be involved in regulating multiple processes, including Wnt signaling.
NCBI, Uniprot Number:
Q53GL7
Package Type:
Plastic Vial
Product Description:
PARP-10 [ARTD10] is a mono-ADP-ribosyltransferase and was initially identified as an interaction partner of the proto-oncoprotein Myc. PARP-10 possesses ubiquitin-interaction motifs, which are important to inhibit the activation of NF-kappaB and downstream target genes in response to interleukin-1beta [IL-1beta] and tumor necrosis factor-alpha [TNF-alpha]. PARP-10 mono(ADP-ribosyl)ates (MARylates) NEMO (NF-kappaB essential modulator), which results in reduced NEMO polyubiquitylation and thus decreased NF-kappaB signaling. Overexpression of PARP-10 was shown to lead to apoptosis. PARP-10 knockdown leads to increased cell survival. Additional substrates of PARP-10, such as GSK-3beta were identified and PARP-10-mediated mono(ADP-ribosyl)ation (MARylation) was shown to be involved in regulating multiple processes, including Wnt signaling.
Specificity:
Recognizes human PARP-10 [ARTD10] with an epitope between aa 256-407, containing the glycine rich (G-rich) region.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibodies
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 year after receipt when stored at +4°C.

References

PARP-10, a novel Myc-interacting protein with poly(ADP-ribose) polymerase activity, inhibits transformation: M. Yu, et al.; Oncogene 24, 1982 (2005) | Dynamic subcellular localization of the mono-ADP-ribosyltransferase ARTD10 and interaction with the ubiquitin receptor p62: H. Kleine, et al.; Cell Commun. Signal. 10, 28 (2012) | Regulation of NF-kappaB signalling by the mono-ADP-ribosyltransferase ARTD10: P. Verheugd, et al.; Nat. Commun. 4, 1683 (2013) | ARTD10 substrate identification on protein microarrays: regulation of GSK3beta by mono-ADP-ribosylation: K.L. Feijs, et al.; Cell Commun. Signal. 11, 1 (2013) | Macrodomain-containing proteins: regulating new intracellular functions of mono(ADP-ribosyl)ation: K.L. Feijs, et al.; Nat. Rev. Mol. Cell Biol. 13, 443 (2013) | Caspase-dependent cleavage of the mono-ADP-ribosyltransferase ARTD10 interferes with its pro-apoptotic function: N. Herzog, et al.; FEBS J. 280, 1330 (2013)