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ScreenCell

ScreenCell® non-invasive technology for isolating Rare Circulating Cells

ScreenCell® is currently the only system, which:

  • is completely independent of any equipment
  • is designed for standardised IVD assays and platforms
  • allows you to filter in only 3 minutes
  • allows the filtering of fixed as well as live cells
  • if needed, allows the analysis of residual fluid
  • is not depending on EpCAM

How can ScreenCell devices help you?

ScreenCell® is a simple and innovative non-invasive technology for isolating Circulating Rare Cells (CRCs) from whole blood, and potentially any atypical cell in biological fluids.

By providing an easy access to fixed or live Circulating Tumour Cells (CTCs) and Circulating Tumour Microemboli (CTMs), the ScreenCell devices allow a full range access to phenotypical, genotypical, and functional characterization of these cells.

ScreenCell devices can also be used to isolate Circulating Foetal Cells (CFCs) in maternal peripheral blood for developing a new non-invasive approach to prenatal diagnosis and potentially leading to a significantly facilitated and cost-effective process for prenatal diagnosis of genetic diseases.

ScreenCell was founded with the objective of designing and producing a simple but revolutionary technology allowing the fast and effective isolating of Rare Circulating Cells yielding free of any bias CTC/CFC population amenable to better cellular and molecular characterization important in cancer monitoring. This technology also contributes to the development of a new non-invasive approach to prenatal diagnosis. ScreenCell designed a full range of devices compatible with existing In vitro Diagnostics (IVD) assays and platforms, with no need for any interface development.

ScreenCell is dedicated to creating technologies allowing CTCs to become potential end points in future oncology therapeutic arsenals, by filtering out healthy live tumour cells, thus enabling the technology to be available for three different applications:

ScreenCell Applications

Molecular Biology

ScreenCell® MB kit is used for molecular biology studies. Each kit contains a set of single use DNAse- and RNAse-free filtration devices individually packed, together with a specific buffer; a collection tube is also included. ScreenCell® MB kits are available for isolating circulating rare cells alive through filtration devices. The ScreenCell MB filtration unit allows either DNA or RNA extraction directly from cells isolated on the filter before or after cell culture (the cupule-filter containing the cultured cells is then transferred with a nuclease-free forceps into a nuclease-free Eppendorf® tube.

Product Code: MB 3LC

Cell Culture

ScreenCell® CC kit is used for cell culture and allows isolation of live cells. The Kit contains a set of single use sterile filtration devices individually packed, together with a specific buffer and a filtration tube. ScreenCell® CC isolation device contains a circular filter, located inside the bottom part of the filtration module and capped by a removable nozzle/holder.

Before filtration, 6 ml blood samples must be diluted in 1 ml of the ScreenCell® LC dilution buffer, followed 2 minutes later by addition of 1.6 ml of adequate tissue culture medium, allowing isolation of live cells.

At the end of filtration, the nozzle/holder of the ScreenCell® CC device is unclipped and removed from the filtration module. The circular filter is then released into a well of a 24 well tissue culture plate, by pushing down a rod located at the bottom part of the filtration device.

Adequate tissue culture medium and growth factors are added into the well. The multiwell plate is then closed and incubated under defined conditions. Cytological studies can then be conducted directly on the filter.

Product Code: CC 3LC-ha

Enumeration and Cytomorphology Evaluation

ScreenCell® CY kit is used for cellular biology studies.

It comprises of a set of single use filtration devices, individually packed, for the isolation of circulating rare cells. A specific buffer and a collection tube is also included.

Before filtration, 3 ml blood samples must be diluted in 4 ml of the ScreenCell® FC filtration buffer for fixed cells. An additional 1.6 ml of 1X PBS is filtered for removing debris from the filter.

At the end of filtration, the nozzle/holder of the ScreenCell® CY device is unclipped and removed from the filtration module. The filter is then released onto a standard microscopy glass slide by pushing down a rod located at the bottom part of the filtration device. Cytological studies such as staining, cell enumeration, immunocytochemistry, FISH assays, etc can be conducted directly on the filter, the filtration area being delimited by a more rigid O ring.

Product Code: CY 4FC

ScreenCell Solutions

ScreenCell® have developed a vast number of validated protocols. Please contact us advising the application that you require the CTC and we can send you the relevant protocols.

Collaborations with the Dana-Farber Cancer Institute and Massachusetts General Hospital, in the USA, Hôpital Saint-Louis, Hôpital Antoine-Béclère, Hôpital Necker and Hôpital de Poissy in France, and others have proven that today ScreenCell devices are best and most user friendly CTC/CFC isolation devices available.

Caltag Medsystems are the exclusive UK distributor for ScreenCell products. For further information please call +44 (0)1280 827460 or email office@caltagmedsystems.co.uk.

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