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StemBioSys

Welcome to StemBioSys - Distributed in the UK & Europe by Caltag Medsystems

The CELLvo matrix is an advanced stem cell culture system that replicates the 3-dimensional "home" in which stem cells naturally reside and proliferate. It contains an extracellular matrix (ECM) of more than 150 proteins synthesized in vitro by bone marrow stromal cells. The final products (6 well plates, T75 and T150 flasks) are cell free and ready to use, consisting of the ECM attached to the surface of the culture vessels.

The CELLvo matrix provides a native three-dimensional microenvironment which can be used for rapid expansion of high quality mesenchymal stem cells. It is the only stem cell substrate to closely mimic the full biochemical and structural microenvironment that stem cells normally see in their native environment.

The CELLvo™ Extracellular Matrix

A Natural Environment for Expanding Stem Cells

Stem cells grown in the CELLvo matrix remain small, proliferative and undifferentiated. Using this unique technology, the isolation and growth of stem cells, from a variety of sources including adipose, bone marrow and umbilical cord blood/tissue is possible. Multiple studies have shown that culture using the CELLvo matrix plates results in a greater quantity of usable stem cells, with a better attachment and a higher quality and motility, when compared to cells grown on tissue culture-treated plastic.

Technical Bulletin:

The CELLvo matrix Reduces Donor-Donor Variability

Unlike protein coated dishes, StemBioSys' High Performance Micro Environment is a natural microenvironment produced by cells for cells. The CELLvo matrix has been shown to allow a greater variety of stem cells to replicate more rapidly and with greater preservation of the stem cell phenotype than traditional tissue culture-treated plastic. Multiple studies performed using the CELLvo matrix have exhibited efficient growth of stem cells, opening up potential for research, diagnostic and clinical uses.

MSCs grown on Bone Marrow - High Performance Micro Environment
MSCs grown on Bone Marrow - High Performance Micro Environment, compared to Tissue Culture Plastic have a shorter doubling time and exhibit less variation in growth potential from donor to donor.

The Microenvironment plays a Significant Role in Gene Expression and Cell Behaviour

Surprisingly, the standard for the isolation and growth of hMSCs from mononuclear cells (MNCs) is to extract them from their natural environment and plate them onto tissue culture-treated plastic (TCP). TCP is a foreign environment for the stem cells which induces a rapid response including a shift in gene expression and ultimately to uncontrolled differentiation. It is becoming more broadly accepted that greater attention must be directed to the substrate on which these cells are cultured. This has led to the development of alternative methods for culture including extracted and purified extracellular matrix (ECM) proteins that can be coated or gelled onto the cell culture surface.1 These substrates still fall well short of mimicking the natural microenvironment from which the cells were extracted.

StemBioSys has developed an advanced stem cell culture system that replicates the 3-dimensional "home" or microenvironment in which stem cells naturally reside and proliferate.2 This unique technology enables users to isolate and grow hMSCs from a variety of sources including but not limited to adipose, bone marrow and umbilical cord blood/tissue.

Gene Heat Map

References

  1. Shakouri-Motlagh, O'Connor, Brennecke. Native and solubilized decellularized extracellular matrix: a critical assessment of their potential for improving the expansion of mesenchymal stem cells. Acta Biomaterialia. 2017.
  2. Lai Y, Sun Y, Skinner C, et al. Reconstitution of marrow-derived extracellular matrix ex vivo: a robust culture system for expanding large-scale highly functional human mesenchymal stem cells. Stem Cells Dev. 2010.

The CELLvo matrix Promotes a Higher Quality and Quantity of Cells

Multiple HPME published studies have demonstrated enhanced proliferation and increased expression of key stem cell characteristics when compared to cells grown on standard tissue culture plastic.

Tissue Culture Plastic
Tissue Culture Plastic
The CELLvo matrix
The CELLvo matrix®

CELLvo Human Cord Blood Endothelial Progenitor Cells (hCB-EPCS)

CELLvo Human Cord - Blood Endothelial Progenitor Cells

CELLvo Human Cord Blood - Endothelial Progenitor Cells. Product Code: EPC-500-000

CELLvo human cord blood endothelial progenitor cells (hCB-EPCS) are progenitors of other endothelial type cells. These cells exhibit superior colony forming abilities, greater vessel formation, and greater angiogenic potential in vitro as well as in vivo when compared to hUVECs. CELLvo™ hCB-EPCs cells are the cell of choice for researchers wanting greater control of their endothelial culture related studies. When grown on the CELLvo™ Matrix, CELLvo™ hCB-EPCs behave more naturally, allowing researchers greater control of their study.

Download the CELLvo™ EPC Whitepaper:

CELLvo Human Bone Marrow Mesenchymal Stem Cells (hBM-MSC)

CELLvo Human Bone Marrow Mesenchymal Stem Cells

CELLvo Human Bone Marrow Mesenchymal Stem Cells. Product Code: BM-100-000

CELLvo human bone marrow mesenchymal stem cells hBM-MSCs have been isolated and cultured under xeno-free conditions on the CELLvo™ Xeno Free Matrix. These cells proliferate more quickly, have a smaller cell size and show a higher percentage of SSEA4 (+) cells when compared to BM-MSCs isolated and cultured on other substrates.

Products

Product Code Description
BM-100-T150 CELLvo Human BMMSCs w/T150 Flask Matrix
BM-100-6WP CELLvo Human BM-MSCs w/6 Well Plate Matrix
BM-100-T75 CELLvo Human BM-MSCs w/T75 Flask Matrix
BM-100-000 CELLvo Human Bone Marrow Mesenchymal Stem Cells
EPC-500-000 CELLvo Human Cord Blood - Endothelial Progenitor Cells
EPC-500-6WP CELLvo Human Cord Blood - Endothelial Progenitor Cells w/6Well Plate Matrix
EPC-5000-T150 CELLvo Human Cord Blood - Endothelial Progenitor Cells w/T150 Flask Matrix
EPC-500-T75 CELLvo Human Cord Blood - Endothelial Progenitor Cells w/T75 Flask matrix
CELLvo Matrix-T-150 CELLvo Matrix, 150cm2 t-flask
CELLvo Matrix-6WP CELLvo Matrix, 6-well plate
CELLvo Matrix-T-75 CELLvo Matrix, 75cm2 t-flask
CELLvo XF Matrix-T-150 CELLvo Xeno Free Matrix, 150cm2 t-flask
CELLvo XF Matrix-6WP CELLvo Xeno Free Matrix, 6-well plate
CELLvo XF Matrix-T-75 CELLvo Xeno Free Matrix, 75cm2 t-flask

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