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StemBioSys

Welcome to StemBioSys - Distributed in the UK and Across Europe by Caltag Medsystems

BM-HPME (Bone Marrow - High Performance Micro Environment) is an advanced stem cell culture system that replicates the 3-dimensional “home” in which stem cells naturally reside and proliferate. It contains an extracellular matrix (ECM) of more than 150 proteins synthesized in vitro by bone marrow stromal cells. The final products (6 well plates, T75 and T150 flasks) are cell free and ready to use, consisting of the ECM attached to the surface of the culture vessels.

The BM-HPME provides a native three-dimensional microenvironment which can be used for rapid expansion of high quality mesenchymal stem cells. It is the only stem cell substrate to closely mimic the full biochemical and structural microenvironment that stem cells normally see in their native environment.

Stem cells grown in BM-HPME remain small, proliferative and undifferentiated. Using this unique technology, the isolation and growth of stem cells, from a variety of sources including adipose, bone marrow and umbilical cord blood/tissue is possible. Multiple studies have shown that culture using the BM-HPME plates results in a greater quantity of usable stem cells, with a better attachment and a higher quality and motility, when compared to cells grown on tissue culture-treated plastic.

Technical Bulletin:

BM-HPME Reduces Donor-Donor Variability

Unlike protein coated dishes, StemBioSys' High Performance Micro Environment is a natural microenvironment produced by cells for cells. BM-HPME has been shown to allow a greater variety of stem cells to replicate more rapidly and with greater preservation of the stem cell phenotype than traditional tissue culture-treated plastic. Multiple studies performed using BM-HPME have exhibited efficient growth of stem cells, opening up potential for research, diagnostic and clinical uses.

MSCs grown on Bone Marrow - High Performance Micro Environment
MSCs grown on Bone Marrow - High Performance Micro Environment, compared to Tissue Culture Plastic have a shorter doubling time and exhibit less variation in growth potential from donor to donor.

The Microenvironment plays a Significant Role in Gene Expression and Cell Behaviour

Surprisingly, the standard for the isolation and growth of hMSCs from mononuclear cells (MNCs) is to extract them from their natural environment and plate them onto tissue culture-treated plastic (TCP). TCP is a foreign environment for the stem cells which induces a rapid response including a shift in gene expression and ultimately to uncontrolled differentiation. It is becoming more broadly accepted that greater attention must be directed to the substrate on which these cells are cultured. This has led to the development of alternative methods for culture including extracted and purified extracellular matrix (ECM) proteins that can be coated or gelled onto the cell culture surface.1 These substrates still fall well short of mimicking the natural microenvironment from which the cells were extracted.

StemBioSys has developed an advanced stem cell culture system that replicates the 3-dimensional "home" or microenvironment in which stem cells naturally reside and proliferate.2 This unique technology enables users to isolate and grow hMSCs from a variety of sources including but not limited to adipose, bone marrow and umbilical cord blood/tissue.

Gene Heat Map

References

  1. Shakouri-Motlagh, O'Connor, Brennecke. Native and solubilized decellularized extracellular matrix: a critical assessment of their potential for improving the expansion of mesenchymal stem cells. Acta Biomaterialia. 2017.
  2. Lai Y, Sun Y, Skinner C, et al. Reconstitution of marrow-derived extracellular matrix ex vivo: a robust culture system for expanding large-scale highly functional human mesenchymal stem cells. Stem Cells Dev. 2010.

BM-HPME Promotes a Higher Quality and Quantity of Cells

Multiple HPME published studies have demonstrated enhanced proliferation and increased expression of key stem cell characteristics when compared to cells grown on standard tissue culture plastic.

Tissue Culture Plastic
Tissue Culture Plastic
BM-HPME
BM-HPME®

Products

Product Code Description Size
BM-HPME-6WP BM-HPME, 6-well plate 5 Plates
BM-HPME-T-75 BM-HPME, 75cm2 t-flask 5 Pieces
BM-HPME-T-150 BM-HPME, 150cm2 t-flask 5 Pieces
XF-HPME- 6WP Xeno Free HPME 6 Well Plate 5 Pieces
XF-HPME-T-75 Xeno Free HPME, 75cm2 t-flask 5 Pieces
XF-HPME-T-150 Xeno Free HPME, 150cm2 t-flask 5 Pieces

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