Custom MHC Tetramers: Directly Measure Antigen-Specific T Cells
MHC Tetramers provide the means to rapidly detect and enumerate antigen specific T cells directly from ex vivo (patient) (donor) samples without the need for cell stimulation.
MBL provide Class I Tetramers, Class II Tetramers, Monomers, and CD1d Tetramers as stock products. For customers wishing to purchase tetramers containing a specific peptide sequence that is not present in the current product portfolio, a custom Tetramer and Monomer service is available (Class I and Class II).
Advantages of MHC Tetramers:
- Class I tetramers from MBL contain the unique proprietary mutation in the alpha-3 domain which reduces non-specific binding
- Immune responses are targeted to the specific allele(s)
- Tetramer staining is quantitative
- Does not require the use of radioactive isotopes
- Rapid : allowing fresh blood (or tissue-derived) sample to be analysed
- Large numbers of samples can be processed using flow cytometer
- Tetramer-labelled cells remain viable
- All specific CTLs are detected
- Tetramers can be customized for unique applications.
Benefits of Custom Tetramers:
- Peptide can be manufactured by MBL or the customer can provide the peptide
- Choice of class I, class II and non-classical MHC alleles available
- Various labels available: APC, PE, FITC, BV421
The process of ordering custom tetramers
MBL Provide a custom tetramer manufacturing service. Why advantages – click here for more info
Two options exist for ordering custom tetramers:
All custom tetramers require a feasibility study (Product Code T05000) to be performed prior to tetramer production.
For MHC Class I tetramers an algorithm-based predication is initially made. This calculates the affinity of the peptide sequence required by the customer, with the specific MHC Class I allele chosen. If the algorithm prediction indicates that successful tetramer production is likely then production of the monomer is undertaken. A HPLC run of the MHC class I/b2 microglobulin/peptide fold then allows determination of whether the peptide has been integrated in the MHC class I/b2 microglobulin complex. If successful, then tetramer production occurs.
For MHC Class II tetramers peptide screening is performed using a HPLC mini-load of peptide/MHC class II. This allows determination of whether the peptide is bound to the MHC class II complex or not. If bound, production of the loaded monomer is undertaken, followed by production of the tetramer.
Option 1) MBL acquire bespoke peptide
In this option, the customer supplies the sequence of the peptide and peptide manufacture is performed as part of the tetramer production run. Customers choosing this option will be charged a peptide acquisition fee. The peptide acquisition fee depends on the MHC Class of the tetramer to be produced. For manufacture of a MHC Class I tetramer please see product code T05008. For manufacture of a MHC Class II tetramer please see product code T05012.
Please click here and fill out this form if you would like to order a custom tetramer and MBL acquire the bespoke peptide.
Option 2) Customer provides bespoke peptide
Here, the customer supplies the relevant peptide. There is no additional fee for this option.
Please click here and fill out this form if you would like to supply the peptide for your custom tetramer order.
Quickswitch™ Tetramer Kits
MBL also offer a new technology for customers to generate new tetramer specificities themselves, perfect for screening peptides before committing to a custom manufacture. Read more here.
If you require any further information regarding tetramers or the custom tetramer ordering process, please do not hesitate to contact firstname.lastname@example.org or call +44 (0)1280 827460.