Peripheral Blood CD3+ Cells

Human T cells are lymphocytes that are generally characterized by the expression of CD3 on the cell surface. These cells originate in the bone marrow, migrate to the thymus to undergo maturation, and are one of the key cell types involved in cell-mediated immunity. T cells destroy invading organisms and aid in the activation of B cells through the thymus-dependent response. There are several different subsets of T cells, including helper T cells (naive and memory), cytotoxic T cells (naive and memory), regulatory T cells, and natural killer T (NKT) cells.
Human peripheral blood CD3+ pan T cells are isolated by means of negative selection. Cells expressing CD14, CD15, CD16, CD19, CD36, CD56, CD123, and CD235a are depleted from the mononuclear cell population using immunomagnetic particles leaving purified, untouched CD3+ pan T cells. Isolated cells are characterized by flow cytometry prior to cryopreservation to ensure a highly pure and viable cell population.
*Our CD3+ Pan T Cells are isolated by means of negative selection. However, we do offer the isolation of CD3+ T cells by positive selection as a custom order. Please inquire to obtain pricing for the positive selection of CD3+ T cells.
Cells are obtained using Institutional Review Board (IRB) approved consent forms and protocols in CLIA Certified clinics registered by the FDA.

Negative