Bio-ReCell - Developing Novel Technologies For Cell Separation And Activation - Distributed in the UK by Caltag
Bio-ReCell
Established in 2018 in Ljubljana, Slovenia, Bio-ReCell was founded to address the specific challenges faced by laboratories and clinics reliant on conventional T-cell separation and activation methods that utilise soluble antibody complexes, ferrous polymeric nano matrices, or paramagnetic beads.
Bio-ReCell's founders recognised the industry's need for reliable alternatives that provide cleaner, residue-free results without proprietary restrictions. Driven by this vision, they developed their patented biocomposite materials with covalently bonded antibodies, allowing precise cell targeting without leaving contaminants or residues. Now, their Atract™ T-Cell Activation Kits directly address the challenges of conventional technologies.
Atract™ T-Cell Activation Kits
Based on their patented technology, Bio-ReCell’s Atract™ Kits enable robust and reliable activation of T cells as a prerequisite for the development of state-of-the-art therapeutics and other innovative solutions.
The Atract™ Kit mimics the role of antigen-presenting cells in a native setting by stimulating naïve T cells via the CD3 subunit of the TCR receptor and invigorating the activation with costimulatory signals specific to T cells. This results in high proliferation rates, improving process efficiency and lowering the cost of cell activation.
The Atract™ Kit is compatible with a range of culturing platforms, including flasks, bags, plates and various sizes of G-Rex® containers. T-cell activation with the Atract™ Kit yields high expansion rates and excellent cell viability, even when used with different activation media.
Atract™ T-Cell Activation Kit V1
Functionalised with anti-CD3 and anti-CD28 antibodies for effective T-cell activation and expansion.
Atract™ T-Cell Activation Kit V2
Functionalised with anti-CD3, anti-CD28, and anti-CD2 antibodies, designed to establish a superior proliferative capacity of stimulated cells.
Why Atract™ Kit Outperforms Conventional T-Cell Activation Technologies
Atract™ Kit is designed to provide clean, complete dissociation following T-cell activation, leaving no unwanted impurities in your workflow and no detectable residual antibodies on the cell surface (below LLOQ/LOD). Unlike conventional activation reagents, Atract™ particles cannot be phagocytosed, as they are approximately 5 times larger than cells. This provides a significant advantage as the particles can be easily removed from the culture using standard filtration after stimulation. The result is a cleaner, chemically defined product profile that reduces a key source of variability and regulatory concern. This residual-free approach requires no magnetic components or specialised hardware, eliminating the need for additional cleaning steps and supporting simpler, more cost-effective processes.
Atract™ Kit also delivers strong and consistent biological performance. Both internal data and independent evaluations show that Atract™ Kit outperforms competitors in total T-cell yield and Chimeric Antigen Receptor (CAR) expression levels.
Functional outcomes further differentiate Atract™ Kit. The system maintains robust activation (CD25/CD69) while delivering comparable expansion, viability, and scalability in closed systems such as G-Rex®. It also delivers enhanced cytotoxic performance, promotes a more favourable CD8-dominant profile, sustained granzyme B production, and reduced cytokine release. In addition, transduction efficiency was shown to be 1.3x higher than competitors across ALL samples, as independently validated by the National Institute of Chemistry and the University of Ljubljana.
In summary, Atract™ Kit combines higher CAR-T yields, cleaner product profiles, and improved functional performance, while simplifying manufacturing workflows and supporting a cleaner path to regulatory compliance.
Atract™ T-Cell Activation Kit Key Features
- Magnet-free activation
- No residuals - no downstream cleaning required
- Non-phagocytisable particles - biocompatible composites with covalently bound antibodies
- Simple and scalable protocol that’s easy to integrate, no special equipment required
- Highly efficient CAR transduction
- Great for T-cell assays with no debeading needed
- High and consistent T-cell activation frequency, with over 80% CD25+ T cells after 48h
- High proliferation and expansion across flasks, bags, plates and G-Rex® platforms