DIANA Biotechnologies - Experts in PCR & Other Molecular Biology Reagents - Distributed in the UK & Ireland by Caltag
DIANA Biotechnologies
DIANA Biotechnologies is a Czech biotech company producing top-quality molecular biology reagents and enzymes for R&D and diagnostics. Powered by a team of top scientists in chemistry, biochemistry and molecular biology, their strong PCR diagnostics background enables the creation of a constantly expanding, broad portfolio of unique PCR and RT-PCR products. All DIANA's products are manufactured in compliance with ISO 13485 standards, ensuring the highest quality and safety in production.
See the Products Available:
DIANA's Technology
DBdirect™
Elevate your (RT)-PCR with DBdirect™ technology! All of DIANA's PCR and RT-PCR mixes can streamline your workflow with reduced handling time and costs, while delivering high-quality and reproducible results.
DBdirect™ technology enables direct PCR from a wide range of biological samples without the need for NA isolation. The key innovation lies in the unique PCR mix formulation, which facilitates direct lysis during the PCR reaction.
Some sample types benefit from a preincubation step in DB Lysis Buffers, followed by the addition of the lysate to the PCR mix.
SuperSens
SuperSens is a superior antibody hot-start method that ensures minimal background amplification and enhanced specificity, making it ideal for complex or low-copy targets. With SuperSens, you get faster activation, exceptional sensitivity and accuracy, greater stability, and precision in every reaction.
DBdirect™ PCR & qPCR mixes SuperSens 2.0
DB PCR Mixes 2.0 are optimised for universal use and feature the DBdirect™ technology for direct PCR without DNA extraction and the SuperSens technology for superior robustness, sensitivity and specificity. Their single-component formulation ensures maximum simplicity. Enjoy reduced hands-on time, consistent results, and complete coverage of your PCR needs – from routine detection to ultrasensitive assays.
Key Features:
- DBdirect™: No need for NA extraction
- Wide range of applications
- SuperSens: Unmatched sensitivity with antibody hot-start
- Exceptionally robust and sensitive
RT-PCR & RT-qPCR Mixes
DB RT-PCR Mixes and DBdirect™ RT-PCR Mixes are the ultimate solution for efficient and reproducible analysis. DIANA's mixes are designed to meet your high standards for quality and performance with exceptional accuracy provided by the SuperSens technology. All the 2.0 mixes are based on the proprietary enzymes, DBscript Reverse Transcriptase and DB AptaTaq DNA Polymerase, supported by DB RNase Inhibitors.
Key Features:
- DBdirect™: No need for NA extraction
- Wide range of applications
- SuperSens: Unmatched sensitivity with our antibody hot-start
- Exceptionally robust and sensitive
Probe or SYBR? Choose the best for your purposes.
DBscript Reverse Transcriptase
DBscript Reverse Transcriptase ensures excellent cDNA synthesis from RNA templates in both one-step and two-step RT-PCR applications.
With a superfast protocol and reduced pipetting steps, it features an MMLV mutant with reduced RNase H activity for improved yield and longer cDNAs (> 9 kb). DBscript supports reverse transcription at 50-60°C, thanks to increased thermostability and a unique buffer formulation. Expressed as a recombinant protein in E. coli, it comes with a 5X RT buffer, including dNTPs and a separate DTT solution.
Key Features:
- Superfast Protocol – Reduced pipetting steps for streamlined workflows
- Wide range sensitivity – Analyse 1 µg to 1 pg of RNA with 2-step RT-PCR
- High-Temperature Stability – Reverse transcription at 50-60°C due to increased thermostability and unique buffer formulation
- Complete Kit – Supplied with 5X RT buffer, including dNTPs and a separate DTT solution
DB Taq DNA Polymerases
DIANA's proprietary DNA Polymerases provide superior thermostability, sensitivity and simplicity with minimal pipetting steps, ensuring reliable results for every experiment.
DB AptaTaq DNA Polymerase
Aptamer-based reversible inhibition simplifies preparation of PCR mixes at ambient temperature and provides high sensitivity and specificity.
DB SuperSens Taq DNA Polymerase
Ultra-efficient proprietary antibody-mediated hot-start ensures superior PCR specificity and sensitivity even in complex DNA mixtures and multiplexed detection.
Key Features:
- High thermostability – Half-life of over 40 min at 95°C
- Highly sensitive and specific hot-start
- Three enzymatic Activities – Includes 5'→3′ polymerase activity, 5'→3′ exonuclease activity and 5′ flap activity.
- Wide range of applications – qPCR from cDNA, genomic DNA, plasmid DNA or synthesis of DNA templates for T/A cloning.
Do you need a ready-to-load option? Make sure to choose a "Green" variant of the polymerases!
DB RNase Inhibitors
RNase inhibitors are the only way to protect RNA across a wide range of applications. However, all protein inhibitors are highly unstable, while synthetic inhibitors have low potency and low assay compatibility. As a result, none of them reliably protects RNA.
DB Ultima H RNase Inhibitor
DB Ultima H RNase inhibitor is designed to solve these issues. DB Ultima H is a highly engineered human RNase inhibitor with an optimal inhibition profile and outstanding stability. It is the most potent inhibitor of human RNases and also potently inhibits other eucaryotic and E.coli RNases. It sets a new benchmark in reliability through unprecedented oxidative resistance and stability across a wide range of assay conditions. DB Ultima H strongly outperforms other inhibitors in direct RT-PCR, RNA-seq, in vitro transcription and other applications.
Key Features:
- Unique storage stability – stable at room temperature in usual PCR buffers for months.
- Exceptional oxidative resistance – retains activity without DTT. Fully active even at 2% H2O2.
- Exceptional thermal stability – the only protein RNase inhibitor stable above 55 °C, enabling increased yields and sensitivity.
DB RNase Inhibitor Bovine
DB RNase Inhibitor Bovine offers potent inactivation of ribonucleases to protect your samples. Designed to overcome common stability challenges, it ensures efficient RNA protection through increased oxidative resistance compared to inhibitors from other species.
DBscript cDNA Synthesis Kit
DIANA's ready-to-use cDNA synthesis Kit provides all the components required for efficient and reliable cDNA preparation from RNA templates. This kit features DIANA's unique DB RNase inhibitor bovine and DBscript Reverse Transcriptase, an MMLV mutant designed for higher yield and synthesis of longer cDNAs (> 9 kb).
Enjoy superior performance with reverse transcription at 50-60 °C, made possible by enhanced protein thermostability and an exclusive buffer formulation. Benefit from an ultra-fast 10-minute protocol for two-step RT-PCR and optimised buffers for seamless one-step RT-PCR.
Key Features:
- Reverse transcription at temperatures from 50 to 60 °C
- Broad sensitivity – Analyse 1 µg to 1 pg of RNA with 2-step RT-PCR
- Short protocol – 10 minutes for two-step RT-PCR
Supplementary Reagents
DB Lysis Buffers
DB Lysis Buffer A is a handy tool for easy and straightforward extraction of gDNA for your application. It is suitable for a wide range of samples, and you can use it together with DIANA's DBdirect™ PCR Mixes.
- Applications: cDNA synthesis, two-step RT-qPCR/RT-PCR
- Quick and straightforward application
- Great solution for single-cell detection
DB Lysis Buffer B is a solution for fast and easy extraction of total RNA from cells and can be used together with DB and DBdirect™ RT-PCR Mixes.
Primers for cDNA Synthesis
Primers are commonly used in cDNA synthesis to initiate the reverse transcription process. Mix DIANA's range of primers: DB Oligo(dT)20 primers, DB Anchored Oligo(dT)20-VN primers or DB Random Hexamers to ensure the best accuracy and efficiency for your cDNA synthesis applications (cDNA synthesis, two-step RT-qPCR/RT-PCR).
AF Fluorescent Dyes
DIANA's AF fluorescent dyes offer exceptional brightness, photostability, and versatility for your research. With an identical structure to Alexa Fluor dyes, they provide the same spectral properties, quality, and reliability for a wide range of fluorescence-based applications. Whether you need precise antibody labelling, confocal and super resolution microscopy or sensitive flow cytometry detection, their AF dyes ensure complete compatibility with existing protocols.
Custom Services
Custom Oligonucleotide Synthesis
Synthetic oligonucleotides are indispensable tools for molecular diagnostics, therapeutics and genetic research. DIANA Biotechnologies delivers top-quality custom DNA and RNA oligonucleotides tailored to your needs. With a wide range of modifications, they guarantee the highest purity, quality, and yield at competitive prices.
Custom Protein Synthesis
Recombinant protein production is essential for academic research and drug discovery. DIANA provides fast and cost-effective protein synthesis services ranging from gene synthesis to up-scale recombinant protein expression and purification at highly competitive prices.
Custom mRNA Synthesis
Synthetic messenger RNA (mRNA) has emerged as an attractive new technology for drug therapy and vaccine development. DIANA Biotechnologies offers a comprehensive solution to mRNA production, ranging from synthesis, purification and quality control of synthetic mRNA to customisable OEM reagents for mRNA production.