Multiplex Assay Kit for Mannose Binding Lectin (MBL) etc. by FLIA (Flow Luminescence Immunoassay)

Product Code:

LMB480Hu

Product Group:

Multiplex Kits

Regulatory Status:

RUO

Target Species:

Human

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Code	Size	Price

LMB480Hu-96T

96T

£498.00

Quantity:

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Further Information

Application:

FLIA Kit for Antigen Detection.

Assay length:

Assay procedure summary:

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100uL standard or sample to each well,
add 10uL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100uL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100uL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100uL sheath solution, swirl for 2 minutes, read on the machine.

Detection range:

0.01-10ng/mL

Item Name:

Mannose Binding Lectin

Method:

Double-antibody Sandwich

Precision:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mannose Binding Lectin (MBL) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mannose Binding Lectin (MBL) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Sample type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Sensitivity:

The minimum detectable dose of this kit is typically less than 0.003 ng/mL

Specificity:

This assay has high sensitivity and excellent specificity for detection of Mannose Binding Lectin (MBL) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Mannose Binding Lectin (MBL) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Stability:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Test principle:

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.

Uniprot:

P11226

Documents

Manual