ProSci

Mouse Normal Tissue Blot II

Product Code:
PSI-1562
Supplier:
ProSci
Host Type:
Mouse
Regulatory Status:
RUO
Application:
Western Blot (WB)
Shipping:
Room Temp
Storage:
Store at 4°C. The blot can be reused after stripping.
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Coomassie blue staining of mouse tissue lysates in 10% SDS-PAGE. Lanes: 1, protein markers; 2, adrenal; 3, cerebellum; 4, cerebrum; 5, small intestine; 6, skeletal muscle; 7, stomach; 8, testis; and 9, thymus.
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Coomassie blue staining of mouse tissue lysates in 10% SDS-PAGE. Lanes: 1, protein markers; 2, adrenal; 3, cerebellum; 4, cerebrum; 5, small intestine; 6, skeletal muscle; 7, stomach; 8, testis; and 9, thymus.

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This product comes from: United States.
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Further Information

Application Note:
For Western Blot, the blot can be blocked with 1% BSA or 5% nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
Background:
The mouse tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different mouse tissue lysates with 15 ?g of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane.The mouse tissues included in the blot are adrenal, cerebellum, cerebrum, small intestine, skeletal muscle, stomach, testis, and thymus.
Diagnosis:
Normal
Gel:
Gradient 4-20%
Quantity Loaded per Lane:
15 ug
Samples Loaded:
Lanes: 1, protein markers; 2, adrenal; 3, cerebellum; 4, cerebrum; 5, small intestine; 6, skeletal muscle; 7, stomach; 8, testis; and 9, thymus.
USDA Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures. Also, for further information on the biosafety classification of human etiologic agents, please consult the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention's Office of Health and Safety (www.cdc.gov/od/ohs).
User Note:
Lysate lane order may vary; please consult accompanying shipment paperwork.

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