Applied Biological Materials (abm)

Human Cutaneous Angiosarcoma Cell Line (KU-CAS3)

Product Code:
ABM-T8028
Product Group:
Cell Lines
Host Type:
Human
Regulatory Status:
RUO
 

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Further Information

Description:
The KU-CAS3 cell line is a novel human cutaneous angiosarcoma model derived from scalp primary angiosarcoma tissue. KU-CAS3 shows endothelial cell properties, with cobblestone appearance at confluence. These cells are CD31 positive, display tube forming activity, actively uptake LDL, express vWF, show high levels of adrenergic β2 receptor, VEGF1 and VEGF2 receptors. STR profiliing was used for cell line authentication. These cells have tumorigenic potential in vivo and are suitable for cutaneous angiosarcoma research. Additional cell lines from this panel:Cat. T8029 - Human Cutaneous Angiosarcoma Cell Line (KU-CAS5)
Donor History:
Male, 73, Korean, Scalp Primary Angiosarcoma
Growth Conditions:
PriCoat™ T25 Flasks (G299) coated with Gelatin Coating Solution (0.1%) (TM063) are required for optimal cell adhesion and growth. The base medium for this cell line is Prigrow X Series Medium (TM8028) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO2.Note: Cells are sensitive to trypsin; Gentle Dissociation Solution (TM080) is recommended for subculture procedures. Morphology will change with contact inhibition; subculture cells when 60-70% confluent. 
Growth Properties:
Adherent, Polygonal; Morphology changes to cobblestone with contact inhibition.
Markers:
CD31, Adrenergic β2 receptor positive, VEGF1 and VEGF2 receptor, vWF, Claudin-5 
Population Doubling:
34
Seeding Density (/cm^2):
7000-8000
Split Ratio:
01:04
Tissue:
Skin

References

You, H. J., Kim, H. K., Kim, M. S., Lee, Y. H., Chung, J. H., Hwang, N. H., ... & Lee, J. H. (2022). Establishment and characterization of two human cutaneous angiosarcoma cell lines, KU-CAS3 and KU-CAS5. Head & Neck, 44(1), 7-17. https://doi.org/10.1002/hed.26886