Applied Biological Materials (abm)

Stable GS-3A4-HepG2 Cell Line

Product Code:
 
ABM-T6016
Product Group:
 
Cell Lines
Host Type:
 
Human
Regulatory Status:
 
RUO
 

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CodeSizePrice
ABM-T60161x10^6 cells / 1.0 mlEnquire
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Further Information

Description:
The parental cell line of GS-3A4-HepG2 (HepG2) was derived from a human hepatoma and was transformed with a vector containing the Glutamine Synthetase gene allowing for ammonia metabolism and the 3A4 gene for drug metabolism. This transformation allows for activity in the immortalized cell line that is similar to primary hepatocytes but with a greater ability to metabolise drugs (3A4 activity). This cell line is highly functional in long term culture and therefore ideal for continuous research into liver function and metabolism. Previous studies have explored the cell lines use into bioartificial liver support systems. These systems are new technologies being developed to treat cases of liver damage where function needs to be supported during liver regeneration. The GS-3A4-HepG2 cell line may be particularly useful to metabolism drugs during long term experiments.
Donor History:
Male, 15, White, Hepatocellular carcinoma
Growth Conditions:
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Mix 1:1 PriGrow II (TM002) and PriGrow IV (TM004) + 10% dialyzed FBS + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO2
Growth Properties:
Adherent, epithelial-like
Population Doubling:
13 - 23
Seeding Density (/cm^2):
20,000 - 40,000
Tissue:
Liver

References

Enosawa, S., Miyashita, T., Suzuki, S., Li, X. K., Tsunoda, M., Amemiya, H., Yamanaka, M., Hiramatsu, S., Tanimura, N., Omasa, T., Suga, K., & Matsumura, T. (2000). Long-term culture of glutamine synthetase-transfected HepG2 cells in circulatory flow bioreactor for development of a bioartificial liver. Cell transplantation, 9(5), 711?715. https://doi.org/10.1177/096368970000900520Araki, N., Tsuruoka, S., Wang, N., Hasegawa, G., Yanagihara, H., Ando, H., Omasa, T., Enosawa, S., Nagai, H., & Fujimura, A. (2008). Human CYP3A4-introduced HepG2 cells: in vitro screening system of new chemicals for the evaluation of CYP3A4-inhibiting activity. Xenobiotica; the fate of foreign compounds in biological systems, 38(11), 1355?1364. https://doi.org/10.1080/00498250802468645