Applied Biological Materials (abm)

Stable FUT8 Over-expressing HEK293S GnT I-/- Cell Line

Product Code:
 
ABM-T6010
Product Group:
 
Cell Lines
Host Type:
 
Human
Regulatory Status:
 
RUO
 

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CodeSizePrice
ABM-T60101x10^6 cells / 1.0 mlEnquire
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Further Information

Description:
Human HEK293S GnT I-/- parental cell line was infected with recombinant lentivirus carrying the FUT8 gene in the pLV-CT vector to establish the Stable FUT8 Over-expressing HEK293S GnT I-/- Cell Line. FUT8 gene encodes for the fucosyltransferase 8 enzyme (α1,6-fucosyltransferase) which is involved with the transfer of fucose from GDP-fucose to N-linked type complex glycopeptides. It has been linked that FUT8 can independently fucosylate high mannose N-glycans without GnTI. Manipulated to over express FUT8, these cells can be utilized for studies relating to fucosylation and the involvement of the enzyme.
Donor History:
Embryo
Growth Conditions:
Grow cells in shaker at 140 rpm/min. PriGrow X Series Medium (TM6010) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO2. Selection with 1 ug/ml Puromycin (G264)
Growth Properties:
Suspension, round
Markers:
Fut8
Population Doubling:
24 - 36
Seeding Density (/ml):
300,000
Tissue:
Kidney

References

Yang, Q., & Wang, L. (2016). Mammalian α-1,6-Fucosyltransferase (FUT8) Is the Sole Enzyme Responsible for the N-Acetylglucosaminyltransferase I-independent Core Fucosylation of High-mannoseN-Glycans. Journal of Biological Chemistry J. Biol. Chem., 291(21), 11064-11071. doi:10.1074/jbc.m116.720789