Applied Biological Materials (abm)

GLUT4-GFP Stable CHO Cell Line (CHO-HIRc-mycGLUT4eGFP)

Product Code:
 
ABM-T6498
Product Group:
 
Cell Lines
Host Type:
 
Hamster
Regulatory Status:
 
RUO
 

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ABM-T64981x10^6 cells / 1 mlEnquire
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Further Information

Description:
Cells can be used as a visual (GFP) cell-based assay for screening GLUT4 translocation modulator
Donor History:
Female, Adult, Chinese hamster
Growth Conditions:
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Ham's F-12 Medium + 10% FBS + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO2. ug/ml Geneticin/G418 (G271) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions.
Growth Properties:
Adherent, epithelial-like
Markers:
GFP, GLUT4
Seeding Density (/cm^2):
8,000 - 10,000
Split Ratio:
1:4 or 1:5
Tissue:
Ovary

References

Komakula SB, Tiwari AK, Singh S. A novel quantitative assay for analysis of GLUT4 translocation using high content screening. Biomed Pharmacother. 2021 Jan;133:111032. doi: 10.1016/j.biopha.2020.111032. Epub 2020 Dec 1. PMID: 33378945.Vijayakumar, M. V., Singh, S., Chhipa, R. R., & Bhat, M. K. (2005). The hypoglycaemic activity of fenugreek seed extract is mediated through the stimulation of an insulin signalling pathway. British journal of pharmacology, 146(1), 41?48. https://doi.org/10.1038/sj.bjp.0706312Vijayakumar, M. V., Ajay, A. K., & Bhat, M. K. (2010). Demonstration of a visual cell-based assay for screening glucose transporter 4 translocation modulators in real time. Journal of biosciences, 35(4), 525?531. https://doi.org/10.1007/s12038-010-0060-0Vijayakumar, M. V., & Bhat, M. K. (2012). Real time qualitative and quantitative GLUT4 translocation assay. Methods in enzymology, 505, 257?271. https://doi.org/10.1016/B978-0-12-388448-0.00022-X