Normal Primary Diploid Mortal NIH Swiss Mouse Embryonic Cells

These primary normal fibroblast like NIH Swiss mouse cells (Cat. T4138) help define the starting normal genetic background of the NIH Swiss mouse diploid cell type. These cells were prepared from fine minces of whole 17 to 19 days old NIH Swiss mouse embryos by the methods of Todaro and Green (ref 2) and grow well in the growth media used to grow all the R/J Model cells.This cell line is part of an eight cell R/J Model of Metastatic Progression designed using the NIH Swiss mice species to acquire isogenic cell types (T4138, T8981,T3317, T8144-T8148) that capture genetic/phenotypic stages in the progression to highly metastatic growth within this same species. Compared to some other models, the metastasis related changes captured in this unique progression model are easier to discern above their less noisy isogenic background and have progressed within their native NIH Swiss in vivo micro-environments. This isogenic R/J Metastasis Cell Model was produced by an in vitro/in vitro six-step progression from mortal normal NIH Swiss mouse cells (Cat. T4138) that were used to produce immortal pre-cancer NIH/3T3 cells (Cat. T8981) that were transfected with the human HRAS oncogene to produce transformed cancer GhrasT-NIH/3T3 cells (Cat. T3317). Then beginning with a series of in vivo/in vitro progressive transfers of GhrasT-NIH/3T3 cells produced primary tumor T1-A cells (Cat. T8144) that produced local metastatic lesion T2-A cells (Cat. T8145) that simultaneously produced twin distant metastatic lung lesion T3-PA cells (Cat. T8146) with its twin distant metastatic liver lesion cells (Cat. T1847). The liver derived cells produced highly distant metastatic lung lesion cells (Cat T1848) which retained the human HRAS oncogene and they demonstrated a highly metastatic phenotype as they produced wide-spread simultaneous external and internal metastases in multiple sites in multiple mice when injected i.v. into nude NIH Swiss mice.Complete list of cells included in the R/J Metastasis Model:Cat. T8981 - NIH-3T3 CellsCat. T4138 - Normal Primary Diploid Mortal NIH Swiss Mouse Embryonic CellsCat. T3317 - HRAS Stably Expressing Transformed NIH/3T3 Cell Line (GhrasT-NIH/3T3)Cat. T8144 - Primary Tumor Cell Line (T1-A )Cat. T8145 - Local Metastasis Tumor Cell Line (T2-A)Cat. T8146 - Distant Pulmonary Metastasis Tumor Cell Line (T3-PA)Cat. T8147 - Distant Hepatic Metastasis Tumor Cell Line (T3-HA)Cat. T8148 - Highly Metastatic Distant Pulmonary Tumor Cell Line (T4-PA)

PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow III (TM003) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO2.Note: avoid excessive alkalinity in media; renew media twice weekly.

Adherent, fibroblast-like

Embryo