Normal Human Primary Hepatic Kupffer Cells

Kupffer Cells, also known as Browicz-Kupffer cells and stellate macrophages, are specialized macrophages located in the liver lining the walls of the sinusoids. Upon activation Kupffer cells release various products like cytokines, prostanoides, nitric oxide and reactive oxygen species. These factors determine the phenotypes of the Kupffer Cells and their neighbouring cells, such as hepatocytes, stellate cells, endothelial cells and other immune cells that travel through the liver. Therefore, kupffer cells are perfect for studying liver's response to infection, toxins, ischemia, resection and other stresses.

Normal liver

PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM5549) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO2.Note: Cells require additional time to recover from post-thaw conditions. Change media every day after the first 48 hours post-thaw, or earlier if media starts to change colour to yellow. Cells are terminally differentiated and can be maintained in culture for up to 7 days. 

Adherent, polygonal

D68, TNF-a, IL-6, CD34, F4/80 Antibody

13,000 - 15,000

Liver