Applied Biological Materials (abm)

Human Primary Chondrocytes

Product Code:
ABM-T0020
Product Group:
Primary Cells
Cell System:
Skeletal Cell System
Cell Type:
Chondrocytes
Species:
Human
Host Type:
Human
Regulatory Status:
RUO
 

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Further Information

Description:
Chondrocytes in vivo produce and maintain the extracellular matrix of the cartilage. In vitro, chondrocytes after serial passages as a monolayer culture have been documented to lose their phenotypic markers and become de-differentiated. Re-differentiation can be induced by culturing the cells in a 3-D culture system. These cells are useful in studying chondrocyte differentiation and effects of cytokines and growth factors on the maintenance of differentiated phenotype.
Donor History:
Normal tissue
Growth Conditions:
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow  X Series Medium (TM0020) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO2.
Growth Properties:
Adherent, multipolar
Seeding Density (/cm^2):
10,000 - 20,000
Tissue:
Cartilage

References

Kim, B. S., Kim, E. J., Choi, J. S., Jeong, J. H., Jo, C. H., & Cho, Y. W. (2014). Human collagen-based multilayer scaffolds for tendon-to-bone interface tissue engineering. Journal of biomedical materials research. Part A, 102(11), 4044?4054. https://doi.org/10.1002/jbm.a.35057