Applied Biological Materials (abm)

Human Primary Alveolar Cells - Type 1

Product Code:
 
ABM-T4003
Product Group:
 
Primary Cells
Cell System:
 
Pulmonary Cell System
Cell Type:
 
Epithelial Cells
Species:
 
Human
Regulatory Status:
 
RUO
 

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ABM-T40035x10? cells / 1.0 mlEnquire
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Further Information

Description:
Type I alveolar epithelial cells extend across several air sacs and cover more than 95% of the alveolar surface area. They are specialized to serve as very thin (often only 25 nm in width) gas-permeable components of the blood-air barrier. The Type I Alveolar cells are the target of many insults, including ventilator-induced lung injury. These cells are useful in investigating the function and pathology of the respiratory system.
Donor History:
Normal tissue
Growth Conditions:
PriCoat™ T25 Flasks (G299) coated with Gelatin Coating Solution (0.1%) (TM063) are required for optimal cell adhesion and growth. PriGrow X Series Medium (TM4003) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO2.Note: change medium every day/other day to remove nonadherent cells and replenish nutrients. Once cells are greater than 70% confluent, change media daily. Cells can be expanded for 3-6 passages using a 1:2 split ratio. 
Growth Properties:
Adherent, polygonal
Markers:
CK-18, CK-19
Population Doubling:
36-48
Seeding Density (/cm^2):
5,000
Split Ratio:
01:02
Tissue:
Lung