Applied Biological Materials (abm)

Prostate Cancer Cell Line (ACRJ-PC28)

Product Code:
 
ABM-T0398
Product Group:
 
Cell Lines
Host Type:
 
Human
Regulatory Status:
 
RUO
 

No additional charges, what you see is what you pay! *

CodeSizePrice
ABM-T03981x10^6 cells / 1.0 mlEnquire
An MTA must be signed before products can be dispatched. Please contact us for more information.
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges to UK mainland customers, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: Canada.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • References
  • Related Products
  • Show All

Further Information

Description:
The ACRJ-PC28 cell line is of neuroendocrine phenotype and does not express prostate-specific genes (AR, NKX3.1, and PSA) at the protein level but showed expression of NE specific markers (SYP and ENO2) at the RNA-Seq level. The cell line is atypical in expressing both basal (CK5 and p63) and luminal (CK18) markers. ACRJ-PC28 lacked expression from the CDKN2Alocus.
Growth Conditions:
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow X Series Medium for T0398 (TM0398) + 5 ng/mL EGF + 50 ug/mL bovine pituitary extract + 100 ng/mL hydrocortisone + 5 ug/mL insulin + 5 ug/mL Apo-Transferin + 0.5 ng/mL recombinant TGFα + 1 umol/L Epinephrine + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO2. 
Growth Properties:
Adherent; flattened to cuboidal after passaging
Immortalisation Method:
Transduction with hTERT and simultaneous suppression of CDKN2A.
Population Doubling:
45 hours
Tissue:
Prostate

References

Valentine, H., Aiken, W., Morrison, B., Zhao, Z., Fowle, H., Wasserman, J. S., ... & Badal, S. (2022). Expanding the prostate cancer cell line repertoire with ACRJ-PC28, an AR-negative neuroendocrine cell line derived from an African-Caribbean patient. Cancer research communications, 2(11), 1355-1371.