Applied Biological Materials (abm)

Immortalized Podocyte Progenitor Cell Line (UM51-Pre-Podo-hTERT)

Product Code:
 
ABM-T0208
Product Group:
 
Cell Lines
Host Type:
 
Human
Regulatory Status:
 
RUO
 

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ABM-T02081x10^6 cells / 1.0 mlEnquire
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Further Information

Description:
The UM51-Pre-Podo-hTERT cell line was derived from progenitor cells isolated from the urine of a 51-year-old African male and immortalized using hTERT. These cells have been shown to proliferate indefinitely while retaining key podocyte characteristics. UM51-Pre-Podo-hTERT cells express several podocyte-specific markers, such as WT1, NPHS1, and SYNPO which are crucial for understanding the differentiation and functional capacity of podocytes. Despite continuous culture, the UM51-Pre-Podo-hTERT line maintains a stable morphology, proliferation capacity, and expression of critical podocyte markers. It also displays contact inhibition and P53 expression, suggesting maintained normal cellular controls. This cell line has been differentiated into mature podocytes and can enter the G0 phase, thus making it suitable for studying podocyte maturation and function, as well as for modeling diseases where podocyte quiescence and re-entry into the cell cycle play a role. Overall, the UM51-hTERT-Pre-Podo line is an excellent model for research studying nephrogenesis and kidney-associated diseases.
Donor History:
51, Male, African, Urine
Growth Conditions:
Use of gelatin coating (TM063) is required for cell adhesion to the culture vessel and optimal proliferation. PriGrow X series medium (TM0208) + + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO2. Note: cells display contact inhibition and should be subcultured when 70% confuence to maintain characteristics. For information regarding cell differentiation, please refer to the Differentiation Protocol PDF under the Documents Tab. 
Growth Properties:
Adherent, cobblestone-like
Immortalisation Method:
Lipofection with the hTERT expressing plasmid
Markers:
 NPHS1, WT1, Resistant to Neomycin
Population Doubling:
48
Split Ratio:
01:10
Tissue:
Kidney

References

Erichsen, L., Klos, L. D. F., Thimm, C., Bohndorf, M., Schichel, K., Wruck, W., & Adjaye, J. (2022). Derivation of the immortalized cell line-UM51-PrePodo-hTERT and its responsiveness to Angiotensin II and activation of RAAS. bioRxiv.Erichsen, L., Thimm, C., Bohndorf, M., Rahman, M. S., Wruck, W., & Adjaye, J. (2022). Activation of the Renin?Angiotensin System Disrupts the Cytoskeletal Architecture of Human Urine-Derived Podocytes. Cells, 11(7), 1095.