Applied Biological Materials (abm)

Immortalized Multimammate Mouse Skin Keratinocytes (Kera5)

Product Code:
ABM-T0763
Product Group:
Cell Lines
Host Type:
Mouse
Regulatory Status:
RUO
 

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Further Information

Description:
Immortalized Multimammate Mouse Skin Keratinocytes (Kera5) are a spontaneously immortalized cell line derived from primary keratinocytes of newborn virus-free Mastomys coucha. Mastomys coucha-derived skin keratinocytes can be used as an in vitro system to investigate molecular and immunological aspects of infectious agent interactions with their host cells.
Donor History:
Newborn virus-free Mastomys coucha
Growth Conditions:
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM0763) + 5 ng/ml human EGF (Z100139) + 1 nM Cholera toxin (Sigma; Cat.No. C8052) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO2
Growth Properties:
Adherent, cobblestone
Immortalisation Method:
Spontaneous immortalization
Markers:
Keratin-14
Tissue:
Skin

References

Hasche, D., Stephan, S., Savelyeva, L., Westermann, F., Rösl, F., & Vinzón, S. E. (2016). Establishment of an Immortalized Skin Keratinocyte Cell Line Derived from the Animal Model Mastomys coucha. PLOS ONE, 11(8), e0161283. https://doi.org/10.1371/journal.pone.0161283